Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed (ab5999)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA, WB
製品の概要
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製品名
Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
IgG + IgM + IgA 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
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アプリケーション
適用あり: Flow Cyt, ICC/IF, ELISA, WBmore details -
吸着処理血清
Human more details -
免疫原
Mouse IgG, IgA, and IgM whole molecules
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Lyophilized:Reconstitute with 1 ml of water. -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/500 - 1/2500.
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| ICC/IF |
1/500 - 1/2500.
|
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| ELISA |
1/10000 - 1/50000.
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| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
1/500 - 1/2500. |
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ICC/IF
1/500 - 1/2500. |
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ELISA
1/10000 - 1/50000. |
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WB
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab5999 は 4 報の論文で使用されています。
- Khaled SAA et al. Quantitative Assay of Mutated Nucleophosmin in Acute Myeloid Leukemia. J Hematol 8:111-120 (2019). PubMed: 32300454
- Hutchinson CV et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). PubMed: 24618035
- Mosiolek M et al. Rumex acetosa Y chromosomes: constitutive or facultative heterochromatin? Folia Histochem Cytobiol 43:161-7 (2005). PubMed: 16201317
- Xia B et al. Versatile fluorescent derivatization of glycans for glycomic analysis. Nat Methods 2:845-50 (2005). PubMed: 16278655