Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab5870)

Submit a review Q&A (6)References (11)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

製品の概要

  • 製品名

    Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 吸着処理血清


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG whole molecule.

  • 標識

    HRP

製品の特性

  • 製品の状態

    Lyophilized:Restore with 500 µL deionized water (or equivalent)

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

    BSA is IgG and protease free
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5870の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/10000.
ELISA
1/60000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.
特記事項
ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/10000.
ELISA
1/60000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (11)

ab5870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5870 は 11 報の論文で使用されています。

  • Lim MY & Bernier NJ Intergenerational plasticity to cycling high temperature and hypoxia affects offspring stress responsiveness and tolerance in zebrafish. J Exp Biol 226:N/A (2023). PubMed: 37497728
  • Petherbridge L  et al. Pre-Clinical Development of an Adenovirus Vector Based RSV and Shingles Vaccine Candidate. Vaccines (Basel) 11:N/A (2023). PubMed: 38006010
  • Baar T  et al. RNA transcription and degradation of Alu retrotransposons depends on sequence features and evolutionary history. G3 (Bethesda) 12:N/A (2022). PubMed: 35253846
  • Lim MY & Bernier NJ Zebrafish parental progeny investment in response to cycling thermal stress and hypoxia: deposition of heat shock proteins but not cortisol. J Exp Biol 225:N/A (2022). PubMed: 36326068
  • Zhang S  et al. Structure of a transcribing RNA polymerase II-U1 snRNP complex. Science 371:305-309 (2021). PubMed: 33446560
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Question

I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


Thanks,

Read More
Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Thank you for your information in the previous email. I have an enquiry about the secondary antibody-HRP. What's the difference between ab5887 and ab5870?

Read More
Answer

Thank you for your question. Both antibodies are only a F(ab)2 Fragment of antibody itself (therefore these antibody will not give F(c) mediated binding), but they different in the immunogens used and therefore epitopes recognized: Ab5887 was raised against Mouse IgG F(ab)2 fragment. Ab5870 was raised against Mouse IgG whole molecule. I hope this information will help. Please do not hesitate to contact us if you need further assistance.

Read More

Question

May I ask once more? My samples are sheep proteins. I am going to use two antibodies (anti GAPDH and anti GDF8 antibodies). The first antibodies are raised in mouse and rat (mouse IgG and Rat IgG), respectively. There are many secondary antibodies (HRP conjugated) which recognize mouse IgG or rat IgG (ex. ab5870, ab5879, ab5887, ab6728.....). As some of them are raised in goat, I am afraid that I will have a high background. I would like to know which secondary antibodies against mouse and rat IgG is good for western blotting to detect sheep proteins. Thank you for your helps.

Read More
Answer

Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I would like to recommend the following: ab6728 Mouse IgG antibody (ab6728) HRP conjugated Rabbit ab6820 Mouse IgG antibody (ab6820) HRP conjugated Donkey Both antisera have been raised in rabbit or donkey and are highly unlikely to cross react against sheep. We have also received favourable reviews for them both.

Read More

Question

Thank you for your info. As I mentioned in my email, I used the same kind secondary antibody from Pierce to compare with your product. It showed a nice signal at dilution 1:20000 whereas your product showed a similar signal at dilution 1:100 under the same experiment condition! I bought your product for a direct ELISA, but it didn't work. That's the reason why I did this control test to coat primary antibody directly to the plate. If you believe it just a bad vial, send me a replacement and I will try the test again.

Read More
Answer

Thank you for your clarification and for sharing more details with us. Certainly, we can offer you a replacement vial - free of charge. Would you be so kind to confirm your Purchase Order Number, or Abcam Order Number. We look forward to hearing from you soon.

Read More

Question

1. Please describe the problem (high background, no signal, non-specific colour development, poor standard curve etc). Signal is weaker than normal antibody. 2. What type of ELISA are you performing? (Direct ELISA, indirect ELISA, sandwich ELISA etc.) Direct ELISA. 3. On what material are you testing the antibody in ELISA? Species? Cell type? etc? Coat the plate with primary Antibodies. 3. How did you coat the plates? Coating buffer: 20mM Tris-HCL pH8.5 100ul/well, primary Ab 1:1000. 4. How did you block the unspecific binding sites? For how long? How did you wash the wells? 3% BSA in TBS, 4 hours, wash by TBST buffer 5X between each step. 5. Primary antibody Specification (in which species was it raised against)? rat anti-HA, mouse anti-BDG At what dilution(s) have you tested this antibody? 1:1000, 1:500 Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 2 hours, 5X TBST 6. Secondary antibody What secondary antibody are you using? Your ab6120-250, ab5870-250. Specification (in which species was it raised against)?goat At what dilution(s) have you tested this antibody? Incubation, wash steps? 1 hour, 5XTBST Do you know whether the problems you are experiencing come from the secondary? I also used second antibody from Pierce as a control, they worked very well. 7. Which detection system did you use? Substrate? HRP, OPD from Sigma 8. Did you apply positive and negative controls along with the samples? Please specify. What did you use for standards? These are control tests. 9. Optimization attempts How many times have you tried the ELISA? Twice. Do you obtain the same results every time? Yes. What steps have you altered? No.

Read More
Answer

Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working. Therefore, at this stage, we would suggest that there is either a problem with the vial you received, or modifications to your protocol are needed to obtain a positive result. You have mentioned in your e-mail that you performed direct ELISA. For this type of ELISA, you need to coat the well with the antigen rather than with the primary antibody. For coating buffer, we would suggest using 0.1 M bicarbonate buffer (pH 9.2) instead of Tris buffer. It stabilizes coated proteins by maintaining their tertiary three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. We hope you find this information useful.If you need anything further or any help then please let us know.

Read More

Question

1. Please describe the problem (high background, no signal, non-specific colour development, poor standard curve etc). Signal is weaker than normal antibody. 2. What type of ELISA are you performing? (Direct ELISA, indirect ELISA, sandwich ELISA etc.) Direct ELISA. 3. On what material are you testing the antibody in ELISA? Species? Cell type? etc? Coat the plate with primary Antibodies. 3. How did you coat the plates? Coating buffer: 20mM Tris-HCL pH8.5 100ul/well, primary Ab 1:1000. 4. How did you block the unspecific binding sites? For how long? How did you wash the wells? 3% BSA in TBS, 4 hours, wash by TBST buffer 5X between each step. 5. Primary antibody Specification (in which species was it raised against)? rat anti-HA, mouse anti-BDG At what dilution(s) have you tested this antibody? 1:1000, 1:500 Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 2 hours, 5X TBST 6. Secondary antibody What secondary antibody are you using? Your ab6120-250, ab5870-250. Specification (in which species was it raised against)?goat At what dilution(s) have you tested this antibody? Incubation, wash steps? 1 hour, 5XTBST Do you know whether the problems you are experiencing come from the secondary? I also used second antibody from Pierce as a control, they worked very well. 7. Which detection system did you use? Substrate? HRP, OPD from Sigma 8. Did you apply positive and negative controls along with the samples? Please specify. What did you use for standards? These are control tests. 9. Optimization attempts How many times have you tried the ELISA? Twice. Do you obtain the same results every time? Yes. What steps have you altered? No.

Read More
Answer

Thank you for getting back to us and providing more details of your protocol. We are very sorry to hear that you are not happy with our product. We have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working. Therefore, at this stage, we would suggest that there is either a problem with the vial you received, or modifications to your protocol are needed to obtain a positive result. You have mentioned in your e-mail that you performed direct ELISA. For this type of ELISA, you need to coat the well with the antigen rather than with the primary antibody. For coating buffer, we would suggest using 0.1 M bicarbonate buffer (pH 9.2) instead of Tris buffer. It stabilizes coated proteins by maintaining their tertiary three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. We hope you find this information useful.If you need anything further or any help then please let us know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com