Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed (ab5879)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHC
製品の概要
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製品名
Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse, and Human Serum Proteins.
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アプリケーション
適用あり: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHCmore details -
吸着処理血清
Cow, Horse, Human more details -
免疫原
Mouse IgG F(c) whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
F(ab')2 Anti-Mouse IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項（標識）
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5879の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/250 - 1/1000.
|
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| WB |
1/500 - 1/2000.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/100000.
|
|
| Electron Microscopy |
Use at an assay dependent concentration.
|
|
| IHC |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
1/250 - 1/1000. |
|
WB
1/500 - 1/2000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/100000. |
|
Electron Microscopy
Use at an assay dependent concentration. |
|
IHC
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab5879 は 7 報の論文で使用されています。
- Ou S et al. S100A16 suppresses the proliferation, migration and invasion of colorectal cancer cells in part via the JNK/p38 MAPK pathway. Mol Med Rep 23:N/A (2021). PubMed: 33355370
- Liu H et al. Dendritic cell-derived exosomal miR-494-3p promotes angiogenesis following myocardial infarction. Int J Mol Med 47:315-325 (2021). PubMed: 33416108
- Li Y et al. Overexpression of G3BP1 facilitates the progression of colon cancer by activating ß-catenin signaling. Mol Med Rep 22:4403-4411 (2020). PubMed: 33000280
- Pu J et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett 12:585-590 (2016). IHC-P ; Human . PubMed: 27347184
- Li H et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). PubMed: 26446566
- Yoshikawa T et al. Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection. PLoS One 5:e8729 (2010). WB . PubMed: 20090954
- Froeling FE et al. Organotypic culture model of pancreatic cancer demonstrates that stromal cells modulate E-cadherin, beta-catenin, and Ezrin expression in tumor cells. Am J Pathol 175:636-48 (2009). PubMed: 19608876
