Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
Related conjugates and formulations
製品の概要
-
製品名
Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
-
アプリケーション
適用あり: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
吸着処理血清
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Mouse IgG F(ab)2 fragment
-
標識
Biotin
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
-
精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5886の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/1000 - 1/5000.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
WB |
1/2000 - 1/10000.
|
|
ELISA |
1/20000 - 1/100000.
|
|
IHC |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
特記事項 |
---|
ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
ELISA
1/20000 - 1/100000. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab5886 は 6 報の論文で使用されています。
- Schülke LC et al. Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia. Hum Reprod 39:892-901 (2024). PubMed: 38365879
- Dabel J et al. New perspectives on fertility in transwomen with regard to spermatogonial stem cells. Reprod Fertil 4:N/A (2023). PubMed: 36489201
- Hiort M et al. Testicular Architecture of Men with 46,XX Testicular Disorders of Sex Development. Sex Dev 17:32-42 (2023). PubMed: 36746123
- Di Persio S et al. Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Rep Med 2:100395 (2021). PubMed: 34622232
- Onishi Y et al. Relationship between lamellar sensory corpuscles distributed along the upper arm's deep arteries and pulsating sensation of blood vessels. J Anat 239:101-110 (2021). PubMed: 33527396
- Schneider F et al. Serum and intratesticular inhibin B, AMH, and spermatogonial numbers in trans women at gender-confirming surgery: An observational study. Andrology 9:1781-1789 (2021). PubMed: 34085780