Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed (ab7006)
Key features and details
- Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
製品の概要
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製品名
Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Human -
特異性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins.
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アプリケーション
適用あり: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
吸着処理血清
Cow, Horse, Mouse, Rat more details -
免疫原
Full length native Human IgG (purified).
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7006の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent concentration.
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/200.
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IHC-Fr |
1/50 - 1/200.
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Immunomicroscopy |
1/50 - 1/200.
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Flow Cyt |
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/200. |
IHC-Fr
1/50 - 1/200. |
Immunomicroscopy
1/50 - 1/200. |
Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab7006 は 6 報の論文で使用されています。
- Asashima H et al. Prior cycles of anti-CD20 antibodies affect antibody responses after repeated SARS-CoV-2 mRNA vaccination. JCI Insight 8:N/A (2023). PubMed: 37606046
- Alexandru N et al. Intravenous Administration of Allogenic Cell-Derived Microvesicles of Healthy Origins Defend Against Atherosclerotic Cardiovascular Disease Development by a Direct Action on Endothelial Progenitor Cells. Cells 9:N/A (2020). PubMed: 32059493
- Tiwari A et al. Controlling the Effective Oxygen Tension Experienced by Cells Using a Dynamic Culture Technique for Hematopoietic Ex Vivo Expansion. Curr Protoc Stem Cell Biol 44:2A.11.1-2A.11.13 (2018). PubMed: 29512129
- Tiwari A et al. Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds. Curr Protoc Stem Cell Biol 36:1C.15.1-1C.15.16 (2016). PubMed: 26840223
- Alvarez-Cienfuegos A et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). PubMed: 27345490
- Tiwari A et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). PubMed: 24510768