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Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)

Submit a review Q&A (1)References (15)

Key features and details

  • Goat F(ab) Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

Conjugates logo Related conjugates and formulations

Biotin FITC Texas Red ® TRITC Unconjugated

製品の概要

  • 製品名

    Goat F(ab) Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • 特異性

    No reaction was observed against anti-Papain or anti-Goat IgG F(c).

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 免疫原

    Rabbit IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

    IgG and protease free
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7171の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/20000 - 1/60000.
Electron Microscopy
Use at an assay dependent concentration.
IHC
Use at an assay dependent concentration.
特記事項
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/20000 - 1/60000.
Electron Microscopy
Use at an assay dependent concentration.
IHC
Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (15)

ab7171 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7171 は 15 報の論文で使用されています。

  • Zhang SQ  et al. APOBEC3B expression has prognostic significance in cervical cancer. Int J Clin Exp Pathol 16:48-56 (2023). PubMed: 37033395
  • Fan R  et al. Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats. Evid Based Complement Alternat Med 2022:2112359 (2022). PubMed: 35432561
  • Shaw IW  et al. A role for steroid 5 alpha-reductase 1 in vascular remodeling during endometrial decidualization. Front Endocrinol (Lausanne) 13:1027164 (2022). PubMed: 36465608
  • Nikiforova N  et al. CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry. Membranes (Basel) 11:N/A (2021). PubMed: 34357175
  • Guan Y  et al. Protective multi-target effects of DL-3-n-butylphthalide combined with 3-methyl-1-phenyl-2-pyrazolin-5-one in mice with ischemic stroke. Mol Med Rep 24:N/A (2021). PubMed: 34643246
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

Read More
Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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