Goat F(ab) Anti-Mouse IgG H&L (Biotin) (ab6822)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Goat F(ab) Anti-Mouse IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
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アプリケーション
適用あり: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IFmore details -
免疫原
Mouse IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
特記事項（標識）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot |
Use at an assay dependent dilution.
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| WB |
1/2000 - 1/10000.
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| ELISA |
1/200000.
|
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| IHC |
Use at an assay dependent dilution.
|
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| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ICC/IF |
1/1000 - 1/5000.
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| 特記事項 |
|---|
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/2000 - 1/10000. |
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ELISA
1/200000. |
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IHC
Use at an assay dependent dilution. |
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Immunomicroscopy
Use at an assay dependent dilution. |
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ICC/IF
1/1000 - 1/5000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab6822 は 2 報の論文で使用されています。
- Jiang F et al. Fluorofenidone attenuates paraquat-induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy. Mol Med Rep 23:N/A (2021). PubMed: 33786626
- O'Connor RA et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer. Oncoimmunology 10:1940675 (2021). PubMed: 34290905
