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Goat Anti-Chicken IgY H&L preadsorbed (ab7113)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Chicken IgY H&L preadsorbed
  • Host species: Goat
  • Suitable for: WB, ICC/IF, IHC-Fr, IHC-P, IP, Immunodiffusion, Conjugation, ELISA

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製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L preadsorbed
    IgY 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Chicken

  • 特異性

    No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Fr, IHC-P, IP, Immunodiffusion, Conjugation, ELISAmore details

  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native Chicken IgY (purified).

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7113の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ELISA
1/600000.
特記事項
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ELISA
1/600000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7113 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7113 は 1 報の論文で使用されています。

  • Almada P  et al. Automating multimodal microscopy with NanoJ-Fluidics. Nat Commun 10:1223 (2019). PubMed: 30874553

レビューと Q&A

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Question

Nice talked with you on the phone about the our order 1773Ab. We are so sorry about the results and send the results to you (see attach file). The protocol of Dot blot are following: 3 ul of serum and TK1puri. were dot on the NC membrane and then blocked in TBST with 10 % non-fat milk for 4 hours . The presence or absence of the primary (anti-thymidine kinase1,TK1) antibody (we produced) was incubated at +4 C over night After incubation with a biotinylated secondary antibody (from Abcom 7113 6000x dilution or from Jackson 10000x dilution) was added for one hour at room temperature. The membrane was then incubated in TBS buffer with avidin-HRP-streptavidin (from Amersham). Finaly ECl detection was reaction 1 min and then exposed to X-ray film. The intensity of a single spot on the film was determined by a Laser Densitometer. From the intensity of the rhTK1 . Results: Now we found the higher cross-reaction between Abcom 7113 and serum sample, did not find for the second Ab from Jackson. See fig. We really hepe that you will give an answer soon.. And We aslo found the 7113 Ab without 50% glycerol for stored at -20 C. We are sorry about te results.

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Answer

Thank you for contacting us for technical support and for sending us all this protocol information and an image, it is very useful to understand your problem. The antibody ab7113 has not been tested for DotBlot so we do not know if it will work in this application and we cannot guarantee it will. I think the problem you are having may be due to the blocking: I would recommend trying 5% milk or 5%BSA in TBST to block, only for 1hour, then add the primary and then the secondary antibodies (diluted in TBST only, no milk). This will help remove the non specific binding. I hope this suggestion will help you, please let me know if I can be of further assistance,

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