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Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed (ab6960)

Submit a review Q&A (1)References (5)

Key features and details

  • Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed
  • Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
  • Host species: Goat
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

製品の概要

  • 製品名

    Goat Anti-Chicken IgY H&L (Cy2 ®) preadsorbed
    IgY 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Chicken

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • 吸着処理血清


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native Chicken IgY (purified).

  • 標識

    Cy2 ®. Ex: 489nm, Em: 505nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 10% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.7 moles Cy2 per mole of Goat IgG

  • ポリ/モノ

    ポリクローナル

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6960の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab6960 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6960 は 5 報の論文で使用されています。

  • Okujeni S & Egert U Self-organization of modular network architecture by activity-dependent neuronal migration and outgrowth. Elife 8:N/A (2019). PubMed: 31526478
  • Anastasiades PG  et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun 7:10584 (2016). IHC-FoFr . PubMed: 26843463
  • Stroebele E & Erives A Integration of Orthogonal Signaling by the Notch and Dpp Pathways in Drosophila. Genetics 203:219-40 (2016). PubMed: 26975664
  • Meyer RE  et al. A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant. BMC Genet 11:104 (2010). ICC/IF . PubMed: 21080953
  • Dinger TC  et al. Androgenetic embryonic stem cells form neural progenitor cells in vivo and in vitro. Stem Cells 26:1474-83 (2008). IHC-Fr . PubMed: 18369101

レビューと Q&A

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Question

Hi, I am trying to decide on the best chicken IgY seconday fluorophore label that would give me the least background for cell immunostaining. Any suggestions? I already tried FITC conjugated and it gave me considerable background. thank you, amy

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Answer

All of our products are of the highest standard. If the product states "immunocytochemistry" then it has been tested for ICC and works well. Please refer to the ICC protocol, linked on the right hand side of the Abcam homepage, for a detailed account of how to reduce non-specific background staining.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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