Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
Key features and details
- Goat Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgY
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
製品の概要
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製品名
Goat Anti-Chicken IgY H&L (Biotin)
IgY 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Chicken -
アプリケーション
適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
chicken IgY whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Tripotassium orthophosphate -
Concentration information loading... -
精製度
IgY fraction -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6876の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/3000.
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| Dot blot |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent dilution.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
|
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| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/3000 - 1/30000.
|
| 特記事項 |
|---|
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ICC/IF
1/500 - 1/3000. |
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Dot blot
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/30000. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab6876 は 11 報の論文で使用されています。
- Takeda T et al. Dose-response relationship of pulmonary disorders by inhalation exposure to cross-linked water-soluble acrylic acid polymers in F344 rats. Part Fibre Toxicol 19:27 (2022). PubMed: 35395797
- Abellán-Álvaro M et al. Inhibition of the medial amygdala disrupts escalated aggression in lactating female mice after repeated exposure to male intruders. Commun Biol 5:980 (2022). PubMed: 36114351
- Huijgens PT et al. Silencing and stimulating the medial amygdala impairs ejaculation but not sexual incentive motivation in male rats. Behav Brain Res 405:113206 (2021). PubMed: 33639266
- Huang HT et al. Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein. Sci Rep 11:6551 (2021). PubMed: 33753806
- Doornaert M et al. Xenogen-free isolation and culture of human adipose mesenchymal stem cells. Stem Cell Res 40:101532 (2019). PubMed: 31421383