Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cells) labeling GFP with ab300643 at 1/50 dilution followed by ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in HEK-293T cells transfected with a Firefly Luciferase expression vector containing a GFP tag. The nuclear counter stain is DAPI (blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

ab14734 staining VDAC1 in HEK293 (positive) and HEK293-VDAC1 KO (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated for 2 hours at room temperature with ab14734 at 1µg/ml (shown in green) and ab186735, anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (shown in magenta) and ab323238, anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY Chimeric (shown in yellow) . Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution; ab150084, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution; and ab150175, Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed, at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 293/GFP (human embryonic kidney epithelial cell) cells labeling GFP with ab300643 at 1/50 dilution followed by ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in 293/GFP cells. The nuclear counter stain is DAPI (blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

Flow Cytometry (Intracellular) analysis of 293T transfected with GFP tagged Firefly Luciferase over expression vector labeling GFP (right) with ab300643 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti-Chicken IgY H&L (Alexa Fluor® 647, ab150175) was used as the secondary antibody at a 1/5000 dilution.  Chicken IgY Isotype control. (left)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

Composite multiplex immunofluorescence staining of ab279297, ab317042 and ab308439 staining NeuN, NEFH and Tau (MBD region) in Mouse Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab279297 (shown in green), ab317042 (shown in magenta) and ab308439 (shown in yellow) at 1µg/ml. Cells were then incubated with ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed, ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed and ab150084 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150175 Alexa Fluor® 647 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (red) was ab150175 Alexa Fluor® 647 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-Fr

AbReview40006****

Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)

ab4674 staining GFAP in rabbit eye tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-chicken IgY H&L (ab150175) (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Alex Zagariya.

• Alexa Fluor®

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Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (AB150175)