Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase) (ab5746)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Biotin FITC Texas Red ® TRITC Unconjugated

製品の概要

  • 製品名

    Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Armenian hamster

  • 特異性

    Diminished reactivity will occur with Golden Syrian Hamster IgG.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, IHCmore details

  • 免疫原

    Armenian Hamster IgG whole molecule

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.00952% Magnesium chloride, 0.788% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.

  • 特記事項（標識）

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5746の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
WB
1/500 - 1/2500.
ELISA
1/2000 - 1/25000.
IHC
Use at an assay dependent dilution.
特記事項
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
WB
1/500 - 1/2500.
ELISA
1/2000 - 1/25000.
IHC
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab5746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5746 は 1 報の論文で使用されています。

  • Zhou N  et al. Matrine-induced apoptosis in Hep3B cells via the inhibition of MDM2. Mol Med Rep 15:442-450 (2017). PubMed: 27959389

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

do you have any image staining with this secondary for IHC with BCIP/NBT?

Read More
Answer

Thank you for contacting us.
This secondary antibody can be used for IHC, and the staining detected using the NBT/BCIP substrate ab7468. Although we do not have an image using this specific secondary on file, I have attached an image of AP conjugated secondary with NBT/BCIP (left image, negative control, right stained V. harveyi 639 cells).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com