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Donkey Anti-Sheep IgG H&L (FITC) (ab6896)

Reviews (1)Q&A (3)References (5)

Key features and details

  • Donkey Anti-Sheep IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Suitable for: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 650 HRP Texas Red ® TRITC Unconjugated

製品の概要

  • 製品名

    Donkey Anti-Sheep IgG H&L (FITC)
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details

  • 免疫原

    Full length native Sheep whole (purified).

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 1 ml of water.

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.

  • 特記事項（標識）

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6896の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
特記事項
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab6896 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6896 は 5 報の論文で使用されています。

  • Cui H  et al. Protocol to image and quantify nucleocytoplasmic transport in cultured cells using fluorescent in situ hybridization and a dual reporter system. STAR Protoc 3:101813 (2022). PubMed: 36386872
  • Luo RY  et al. ProBDNF promotes sepsis-associated encephalopathy in mice by dampening the immune activity of meningeal CD4+ T cells. J Neuroinflammation 17:169 (2020). PubMed: 32466783
  • Allen TA  et al. Circulating tumor cells exit circulation while maintaining multicellularity, augmenting metastatic potential. J Cell Sci 132:N/A (2019). PubMed: 31409692
  • Lee CC  et al. Naa10p Inhibits Beige Adipocyte-Mediated Thermogenesis through N-a-acetylation of Pgc1a. Mol Cell 76:500-515.e8 (2019). PubMed: 31422874
  • Holm LJ  et al. Fenofibrate increases very-long-chain sphingolipids and improves blood glucose homeostasis in NOD mice. Diabetologia N/A:N/A (2019). PubMed: 31410530

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Detection of sheep anti tyrosine hydroxylase labelling of splenic nerves

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
I used this antibody to detect sheep anti tyrosine hydroxylase (TH) labelling of splenic nerves in mouse spleen sections along with rat anti-CD35 labelling of follicular dendritic cells (FDC) and rabbit anti PrP labelling of prion protein. It was diluted 1/200 for use on frozen sections that were fixed in acetone and blocked with serum for 30 mins at room temperature. I chose this antibody as I found it difficult to find secondary's that were anti-sheep. I was concerned that the FITC might bleach during confocal microscopy however I was able to scan the triple labelling without any bleaching occurring.

Image is of red CD35+ FDC, blue PrP and green TH+ nerves detected using donkey anti-sheep_FITC
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Laura McCulloch

Verified customer

投稿 Oct 23 2013

Question

We have already ordered antibodies from other company. I just asked my boss. You can refund our money on our account. Nice to contact with you and thank you very much for your help!

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Answer

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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Question

Yes, the image of DAKO gfap staining is the typical astrocyte, just as the image of instruction your company provided to us with ab90601 antibody. So, you can see the significant differences!

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Answer

Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let me know how you would like to proceed.

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Question

to whom may be concerned: our lab ordered four antibodies from your company in July. we cannot find the order list now. ab90601 sheep polyconal to GFAP. ab11267 mouse monoclonal HM-2 to MAP2 - neuronal marker and other two secondary antibodies: ab6896 Donkey polyclonal secondary antibody to sheep IgG - H&L(FITC) and ab47052 Goat polyclonal secondary antibody to Mouse IgG - H&L(AMCA). we used the GFAP to stain astrocyte of rat brain frozen sections and MAP2 to stain neuron of rat brain frozen sections. However, we found the images of GFAP staining were very similar to neurons, clearly not astrocytes. we repeat experiment at least three times and obtain the same results. last time, we borrowed GFAP antibody from other lab ( DAKO company product ) and followed the same procedures and make comparison with your company's GFAP. We obtained typical image of astrocyte by GFAP of borrowed antibody (DAKO product). However, for your company's GFAP, we obtained images similar to neurons just like before. Besides, for the MAP2 of your company, we cannot get any typical images of neurons although we followed the same procedures as doing GFAP. For every experiment, we made negative controls. Finally, we believe there must be somthing wrong with your two primary antibodies(GFAP and MAP2). It cost us more than two weeks to do such experiments which made us very frustrated. We just want to proceed our experiment quickly. so IF possible, someone can check it for us and send us the right antibody quickly so we can continue our experiments.

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Answer

We spoke just a short while ago regarding these issues you were having with ab90601 and ab11267. I have processed the replacement orders as we discussed and sent you an update via email just a few minutes ago. You should receive these 2 replacement antibodies for ab90601 and ab11267 tomorrow. I am sorry for the trouble these 2 antibodies have caused and I do hope the replacements work as expected. Please feel free to follow up with me if you need anything further.

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