AB6900

Donkey Anti-Sheep IgG H&L (HRP)

Name: Donkey Anti-Sheep IgG H&L (HRP) (ab6900) | Abcam
Brand: Abcam Limited
SKU: AB6900
Price: 41000 JPY
Availability: InStock

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(28 Publications)

Suitable for Dot, IM, ICC, WB, ELISA, IHC-P, IHC-Fr. Ideal for western blot. Cited in 28 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA results of purified ab6900 tested against purified Sheep IgG.

Each well was coated in duplicate with 1.0 μg of Sheep IgG. The starting dilution of antibody was 5 μg/ml and the X-axis represents the Log₁₀ of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC₅₀ is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

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Western blot - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

Secondary

All lanes:

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900) at 1/20000 dilution

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Key facts

宿主種

Donkey

ターゲット種

Sheep

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

標識

HRP

励起波長/蛍光波長

アプリケーション

IM, ICC, ELISA, IHC-P, WB, IHC-Fr, Dot

applications

クローン性

Polyclonal

アイソタイプ

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000 - 1/15000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

バッファー組成

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Sheep IgG

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40705007

2025

Coordinated transcriptional control of the adult ventricular conduction system.

Applications

Unspecified application

Species

Unspecified reactive species

Ozanna Burnicka-Turek,Katy A Trampel,Brigitte Laforest,Michael T Broman,Xinan H Yang,Zoheb Khan,Eric Rytkin,Binjie Li,Ella Schaffer,Margaret Gadek,Kaitlyn M Shen,Igor R Efimov,Ivan P Moskowitz

Cells 13: PubMed39451251

2024

AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Nakanishi,Yu-Ichiro Koma,Shoji Miyako,Rikuya Torigoe,Hiroki Yokoo,Masaki Omori,Keitaro Yamanaka,Nobuaki Ishihara,Shuichi Tsukamoto,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Hiroshi Yokozaki,Yoshihiro Kakeji

Journal of tissue engineering and regenerative medicine 2024: PubMed38571695

2024

Genetically engineered macrophages derived from iPSCs for self-regulating delivery of anti-inflammatory biologic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Klimak,Farshid Guilak

The American journal of pathology 194:828-848 PubMed38320632

2024

Periostin in Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression by Enhancing Cancer and Stromal Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shoji Miyako,Yu-Ichiro Koma,Takashi Nakanishi,Shuichi Tsukamoto,Keitaro Yamanaka,Nobuaki Ishihara,Yuki Azumi,Satoshi Urakami,Masaki Shimizu,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Yoshihiro Kakeji,Hiroshi Yokozaki

The Journal of biological chemistry 298:102676 PubMed36336076

2022

The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Seita Tomida,Masamichi Nagae,Yasuhiko Kizuka

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Frontiers in endocrinology 13:960279 PubMed36034417

2022

Dexamethasone-associated metabolic effects in male mice are partially caused by depletion of endogenous corticosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa L Koorneef,Merel van der Meulen,Sander Kooijman,Elena Sánchez-López,Jari F Scheerstra,Maaike C Voorhoeve,Ajith N Nadamuni Ramesh,Patrick C N Rensen,Martin Giera,Jan Kroon,Onno C Meijer

Hepatology communications 6:2689-2701 PubMed35833455

2022

Hepatic patatin-like phospholipase domain-containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Elke Ericson,Linnéa Bergenholm,Anne-Christine Andréasson,Carly I Dix,Jane Knöchel,Sara F Hansson,Richard Lee,Jennifer Schumi,Madeleine Antonsson,Ola Fjellström,Patrik Nasr,Mathias Liljeblad,Björn Carlsson,Stergios Kechagias,Daniel Lindén,Mattias Ekstedt

Experimental and therapeutic medicine 23:277 PubMed35317443

2022

Monomeric C-reactive protein level is associated with osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Liang,Ke Xu,Wenguang Liu,Xiaoling Liu,Ping Yuan,Peng Xu,Haiyun Li

The Biochemical journal 478:3869-3889 PubMed34668531

2021

AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Ju Chuang,Manuel Johanns,Sébastien Pyr Dit Ruys,Gregory R Steinberg,Bruce E Kemp,Benoît Viollet,Mark H Rider

View all publications

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Donkey Anti-Sheep IgG H&L (HRP)