AB6899

Donkey Anti-Sheep IgG H&L (Biotin)

Name: Donkey Anti-Sheep IgG H&L (Biotin) (ab6899) | Abcam
Brand: Abcam Limited
SKU: AB6899
Price: 41000 JPY
Availability: InStock

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(6 Publications)

Donkey Anti-Sheep IgG H&L (Biotin)(ab6899) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (AB6899)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (AB6899)

ELISA results of ab6899 against Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate and TMB substrate.

Key facts

宿主種

Donkey

ターゲット種

Sheep

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

標識

Biotin

励起波長/蛍光波長

アプリケーション

ELISA, IHC-P, ICC/IF, IHC-Fr, Dot, IM, WB

applications

クローン性

Polyclonal

アイソタイプ

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Sheep IgG

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36361905

2022

Characterization of a Bioactive Peptide T14 in the Human and Rodent Substantia Nigra: Implications for Neurodegenerative Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Susan Adele Greenfield,Giovanni Ferrati,Clive W Coen,Auguste Vadisiute,Zoltan Molnár,Sara Garcia-Rates,Sally Frautschy,Gregory M Cole

Glia 67:741-758 PubMed30548340

2018

Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Recasens,Kalpana Shrivastava,Beatriz Almolda,Berta González,Bernardo Castellano

Experimental biology and medicine (Maywood, N.J.) 242:1499-1507 PubMed28766984

2017

Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Davis,Martha C Washington,Emily R Yaniz,Heidi Phillips,Ayman I Sayegh,Megan J Dailey

Experimental biology and medicine (Maywood, N.J.) 242:1490-1498 PubMed28766983

2017

LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Blackmore,Weinan Zhou,Megan J Dailey

Glia 64:2104-2119 PubMed27535761

2016

Astrocyte-targeted production of interleukin-6 reduces astroglial and microglial activation in the cuprizone demyelination model: Implications for myelin clearance and oligodendrocyte maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Filip Petković,Iain L Campbell,Berta Gonzalez,Bernardo Castellano

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:11984-2000 PubMed25186745

2014

Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob Wienecke,Li-Qun Ren,Hans Hultborn,Meng Chen,Morten Møller,Yifan Zhang,Mengliang Zhang

View all publications

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Donkey Anti-Sheep IgG H&L (Biotin)