AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Name: Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155) | Abcam
Brand: Abcam Limited
SKU: AB150155
Price: 30000 JPY
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(171 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 110 publications

別名を表示する

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (red) was ab150155 Alexa Fluor® 647 donkey anti-rat IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

Key facts

宿主種

Donkey

ターゲット種

Rat

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Human

血清吸着処理済み

Yes

標識

Alexa Fluor® 647

励起波長/蛍光波長

Ex: 650nm, Em: 665nm

アプリケーション

Flow Cyt, ICC/IF, IHC-Fr, ELISA, IHC-P

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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製品の詳細

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information

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文献 (171)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1452 PubMed41068278

2025

Immediate-early genes Arc and c-Fos show divergent brain-wide expression following contextual fear conditioning.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas E Bulthuis,Liliette I Quintana,Michelle Stackmann,Christine A Denny

Nature 646:934-944 PubMed40903576

2025

Dynamic fibroblast-immune interactions shape recovery after brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan A Ewing-Crystal,Nicholas M Mroz,Amara Larpthaveesarp,Carlos O Lizama,Remy Pennington,Pailin Chiaranunt,Jason I Dennis,Anthony A Chang,Eric Dean Merrill,Sofia E Caryotakis,Nikhita Kirthivasan,Leon Teo,Tatsuya Tsukui,Aditya Katewa,Gabriel L McKinsey,Sophia C K Nelson,Agnieszka Ciesielska,Nicole C Lummis,Lucija Pintarić,Madelene W Dahlgren,Amha Atakilit,Helena Paidassi,Saket Jain,Xiaodan Liu,Duan Xu,Manish K Aghi,James A Bourne,Jeanne T Paz,Richard Daneman,Fernando F Gonzalez,Dean Sheppard,Anna V Molofsky,Thomas D Arnold,Ari B Molofsky

Aging 17:2089-2112 PubMed40848274

2025

Blood vessel and osteocyte networks concurrently rearrange during bone maturation and decline during aging in the femur of male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mathilde Palmier,Marlène Maître,Hélène Doat,Thierry Lesté-Lasserre,Claudine Boiziau,Delphine B Maurel

Nature communications 16:6779 PubMed40702081

2025

Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuming Wu,Yujing Zhang,Yuan Yu,Xin Wang,Zifan Zhen,Yin Yuan,Bing Xie,Mengqi Han,Mengyuan Wang,Xinyu Zhang,Xueqiang Sun,Xiaoyue Wen,Kenji Hashimoto,You Shang,Shiying Yuan,Jiancheng Zhang

Biomaterials research 29:0226 PubMed40606681

2025

Biomimetic Catechol-Incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary Glands.

Applications

Unspecified application

Species

Unspecified reactive species

Seokjun Kwon,Ji Hyun Ryu,Junchul Kim,Hyun Ho Shin,Gehoon Chung,Ali Taghizadeh,Jung-Hwan Lee,Jongho Kim,Bon-Cheol Ku,Kyungpyo Park,Sang-Woo Lee

Nature cardiovascular research 4:921-937 PubMed40579458

2025

Mechanosensitive PIEZO2 channels shape coronary artery development.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Pampols-Perez,Carina Fürst,Oscar Sánchez-Carranza,Elena Cano,Jonathan Alexis Garcia-Contreras,Lisa Mais,Wenhan Luo,Sandra Raimundo,Eric L Lindberg,Martin Taube,Arnd Heuser,Anje Sporbert,Dagmar Kainmueller,Miguel O Bernabeu,Norbert Hübner,Holger Gerhardt,Gary R Lewin,Annette Hammes

Nature 645:158-167 PubMed40562934

2025

Engrafted nitrergic neurons derived from hPSCs improve gut dysmotility in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Homa Majd,Ryan M Samuel,Andrius Cesiulis,Jonathan T Ramirez,Ali Kalantari,Kevin Barber,Sina Farahvashi,Zaniar Ghazizadeh,Alireza Majd,Angeline K Chemel,Mikayla N Richter,Subhamoy Das,Jacqueline L Bendrick,Matthew G Keefe,Jeffrey Wang,Rahul K Shiv,Samyukta Bhat,Matvei Khoroshkin,Johnny Yu,Tomasz J Nowakowski,Kwun Wah Wen,Hani Goodarzi,Nikhil Thapar,Julia A Kaltschmidt,Conor J McCann,Faranak Fattahi

eLife 13: PubMed40424181

2025

Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Madeline M Keenen,Liheng Yang,Huan Liang,Veronica J Farmer,Rizban E Worota,Rohit Singh,Amy S Gladfelter,Carolyn B Coyne

Molecular medicine (Cambridge, Mass.) 31:119 PubMed40140769

2025

The transcriptional repressor Ctbp2 as a metabolite sensor regulating cardiomyocytes proliferation and heart regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanting Meng,Jianwen Ding,Yanping Wang,Jing Wang,Wei Huang,Wenkang Jiang,Jiayi Li,Xiujuan Lang,Sifan Zhang,Yumei Liu,Xijun Liu,Hulun Li,Bo Sun

Nature communications 16:2845 PubMed40122897

2025

Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Honghui Zheng,Yilin Feng,Jiyuan Tang,Feifei Yu,Zitian Wang,Jiani Xu,Cheng Hai,Mingyue Jiang,Yifan Cheng,Zhicheng Shao,Ning Ma,Peter E Lobie,Shaohua Ma

View all publications

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Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed