Donkey Anti-Rabbit IgG H&L (HRP)

• WB

AbReview46985****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

All lanes:

Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-acetyl-k9-antibody-chip-grade-ab4441'>ab4441</a>) at 1/2500 dilution

Lane 1:

Mouse MEL cell nuclear lysate at 9 µg

Lane 2:

Mouse MEL cell nuclear lysate treated with 0.4 µM Trichostatin A treatment for 18 hours. at 9 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

Predicted band size: 15 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

PubMed

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

Canonical histones are retained in C. elegans sperm.

A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
For full details please see paper.

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

false

Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

AbReview36742****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

true

Exposure time: 1min

This image is courtesy of an Abreview submitted by Min young Kim

• WB

AbReview36742****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

All lanes:

Western blot - Anti-PIAS1 antibody (<a href='/products/primary-antibodies/pias1-antibody-ab32219'>ab32219</a>) at 1/2500 dilution

Lane 1:

293T cell lysate at 25 µg

Lane 2:

MEL cell lysate at 25 µg

Lane 3:

K562 cell lysate at 25 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

true

Exposure time: 1min

This image is courtesy of an Abreview submitted by Min young Kim

• WB

AbReview46986****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

All lanes:

Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-acetyl-k27-antibody-chip-grade-ab4729'>ab4729</a>) at 1/2500 dilution

Lane 1:

Untreated Mouse MEF cell lysate at 9 µg

Lane 2:

0.4 µM Trichostatin A treatment for 18 hr Mouse MEF cell lysate at 9 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

Predicted band size: 15 kDa

false

• WB

AbReview37089****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview.

• WB

Supplier Data

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

Western blot analysis of ab6802 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/1000 dilution

false

• WB

Supplier Data

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB6802)

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

Lane M : 3 μl Molecular ladder.

Lane 1 : Rabbit IgG whole molecule.

Lane 2 : Rabbit IgG F(ab) fragment.

Lane 3 : Rabbit IgG F(c) fragment.

Lane 4 : Rabbit IgM whole molecule.

Lane 5 : Normal rabbit serum.

All samples were reduced. Load : 50 ng per lane. Blocked for 30 min at RT.

ab6802 used at 1 : 5,000 for 60 min at RT. Secondary antibody : None. Predicted/Obsevered Size : 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

false