AB175649

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175649) | Abcam
Brand: Abcam Limited
SKU: AB175649
Price: 37000 JPY
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(36 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 36 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175649, Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed, used at 2μg/ml for 1h at room temperature. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Key facts

宿主種

Donkey

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

血清吸着処理済み

Yes

標識

Alexa Fluor® 405

励起波長/蛍光波長

Ex: 401nm, Em: 421nm

アプリケーション

Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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製品の詳細

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10713 PubMed39715766

2024

Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eirini Bellou,Agata P Zielinska,Eike Urs Mönnich,Nina Schweizer,Antonio Z Politi,Antonina Wellecke,Claus Sibold,Andreas Tandler-Schneider,Melina Schuh

Nature communications 15:9337 PubMed39472435

2024

Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ying Huang,Yan-Zhou Chen,Chuang Zhao,Xin-Nan Zheng,Kai Yu,Jia-Xing Yue,Huai-Qiang Ju,Yan-Xia Shi,Lin Tian

Cell 187:3427-3444.e21 PubMed38733990

2024

Spinal projecting neurons in rostral ventromedial medulla co-regulate motor and sympathetic tone.

Applications

Unspecified application

Species

Unspecified reactive species

Zicong Zhang,Junfeng Su,Jing Tang,Leeyup Chung,Jessica C Page,Carla C Winter,Yuchu Liu,Evgenii Kegeles,Sara Conti,Yu Zhang,Jason Biundo,Joshua I Chalif,Charles Y Hua,Zhiyun Yang,Xue Yao,Yang Yang,Shuqiang Chen,Jan M Schwab,Kuan Hong Wang,Chinfei Chen,Michael J Prerau,Zhigang He

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00346 PubMed38493058

2024

Neuroprotective effects of niclosamide on disease progression via inflammatory pathways modulation in SOD1-G93A and FUS-associated amyotrophic lateral sclerosis models.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Milani,Ilaria Della Valle,Simona Rossi,Paola Fabbrizio,Cassandra Margotta,Giovanni Nardo,Mauro Cozzolino,Nadia D'Ambrosi,Savina Apolloni

Cell reports 43:113801 PubMed38363678

2024

DLK signaling in axotomized neurons triggers complement activation and loss of upstream synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Asghari Adib,Jennifer L Shadrach,Lauren Reilly-Jankowiak,Manish K Dwivedi,Abigail E Rogers,Shameena Shahzad,Ryan Passino,Roman J Giger,Brian A Pierchala,Catherine A Collins

Experimental neurobiology 32:387-394 PubMed38196134

2024

Distinct Role of Parvalbumin Expressing Neurons in the Reticular Thalamic Nucleus in Nociception.

Applications

Unspecified application

Species

Unspecified reactive species

Sanggeon Park,Jeiwon Cho,Yeowool Huh

Science advances 9:eadd6480 PubMed37595032

2023

Organoid cell fate dynamics in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Zheng,Max A Betjes,Pascal Ender,Yvonne J Goos,Guizela Huelsz-Prince,Hans Clevers,Jeroen S van Zon,Sander J Tans

Cells 12: PubMed37048088

2023

Intramuscular IL-10 Administration Enhances the Activity of Myogenic Precursor Cells and Improves Motor Function in ALS Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Fabbrizio,Cassandra Margotta,Jessica D'Agostino,Giuseppe Suanno,Lorenzo Quetti,Caterina Bendotti,Giovanni Nardo

Inflammation and regeneration 43:19 PubMed36895050

2023

Immune-mediated myogenesis and acetylcholine receptor clustering promote a slow disease progression in ALS mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Cassandra Margotta,Paola Fabbrizio,Marco Ceccanti,Chiara Cambieri,Gabriele Ruffolo,Jessica D'Agostino,Maria Chiara Trolese,Pierangelo Cifelli,Veronica Alfano,Christian Laurini,Silvia Scaricamazza,Alberto Ferri,Gianni Sorarù,Eleonora Palma,Maurizio Inghilleri,Caterina Bendotti,Giovanni Nardo

eLife 11: PubMed36445322

2022

Mother cells control daughter cell proliferation in intestinal organoids to minimize proliferation fluctuations.

Applications

Unspecified application

Species

Unspecified reactive species

Guizela Huelsz-Prince,Rutger Nico Ulbe Kok,Yvonne Goos,Lotte Bruens,Xuan Zheng,Saskia Ellenbroek,Jacco Van Rheenen,Sander Tans,Jeroen S van Zon

View all publications

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed