AB182020

Donkey Anti-Rabbit IgG H&L

Name: Donkey Anti-Rabbit IgG H&L (ab182020) | Abcam
Brand: Abcam Limited
SKU: AB182020
Price: 33000 JPY
Availability: InStock

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Suitable for WB, IP, IHC-P, ELISA.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images

ELISA - Donkey Anti-Rabbit IgG H&L (AB182020)

ELISA

Lab

ELISA - Donkey Anti-Rabbit IgG H&L (AB182020)

Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.

Key facts

宿主種

Donkey

ターゲット種

Rabbit

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

アプリケーション

IHC-P, ELISA, WB, IP

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

The antibody reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY, less than 2%.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/200000", "notes":"" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Rabbit IgG

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Donkey Anti-Rabbit IgG H&L