Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)
Key features and details
- Donkey Anti-Mouse IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Dot blot, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IF
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Mouse IgG H&L (PE) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Mouse -
特異性
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
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アプリケーション
適用あり: Dot blot, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Mouse IgG whole molecule
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項(標識)
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7003の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Immunomicroscopy |
Use at an assay dependent concentration.
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Flow Cyt |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/100 - 1/250. |
ICC/IF
1/100 - 1/250. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab7003 は 9 報の論文で使用されています。
- Lee PH et al. Nectin4 is a potential therapeutic target for asthma. Front Immunol 13:1049900 (2022). PubMed: 36457999
- Yu H et al. Strontium ranelate promotes chondrogenesis through inhibition of the Wnt/ß-catenin pathway. Stem Cell Res Ther 12:296 (2021). PubMed: 34016181
- Rajendran Nair DS et al. Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats. Cells 10:N/A (2021). PubMed: 34831174
- Harati R et al. Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling. Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 32645833
- Dai T et al. Analysis of the clinical significance of DCLK1+ colorectal cancer using novel monoclonal antibodies against DCLK1. Onco Targets Ther 11:5047-5057 (2018). PubMed: 30174443
- Banerjee SR et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues. Biomacromolecules 18:201-209 (2017). PubMed: 28001364
- Cheng W et al. Role of the novel HSP90 inhibitor AUY922 in hepatocellular carcinoma: Potential for therapy. Mol Med Rep 12:2451-6 (2015). WB . PubMed: 25955495
- Zhu J et al. Effects of the novel heat shock protein 90 inhibitor AUY922 in renal cell carcinoma ACHN and 786-O cells. Oncol Lett 10:941-945 (2015). WB . PubMed: 26622600
- Marion-Poll L et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt . PubMed: 24713002