Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Mouse -
アプリケーション
適用あり: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISAmore details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175658の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| ICC/IF | (1) |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
| IHC-P |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
|
IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
画像
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)
ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175658 Alexa Fluor® 405 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab175658 は 26 報の論文で使用されています。
- Rahmani W et al. Attenuation of SARS-CoV-2 infection by losartan in human kidney organoids. iScience 25:103818 (2022). PubMed: 35106453
- Chung H et al. Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2. STAR Protoc 3:101617 (2022). PubMed: 35990736
- Kakihana T et al. The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43. iScience 24:102733 (2021). PubMed: 34258561
- Miranda-Barrientos J et al. Ventral tegmental area GABA, glutamate, and glutamate-GABA neurons are heterogeneous in their electrophysiological and pharmacological properties. Eur J Neurosci N/A:N/A (2021). PubMed: 33619763
- Suzuki S et al. Differential RA responsiveness among subsets of mouse late progenitor spermatogonia. Reproduction 161:645-655 (2021). PubMed: 33835049