AB150106

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)

Name: Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150106) | Abcam
Brand: Abcam Limited
SKU: AB150106
Price: 31000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(80 Publications)

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications

別名を表示する

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150106 Alexa Fluor® 555 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

Key facts

宿主種

Donkey

ターゲット種

Mouse

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

血清吸着処理済み

標識

Alexa Fluor® 555

励起波長/蛍光波長

Ex: 555nm, Em: 565nm

アプリケーション

ICC/IF, IHC-Fr, Flow Cyt, ELISA, IHC-P

applications

クローン性

Polyclonal

アイソタイプ

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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製品の詳細

This antibody reacts with the heavy and light chains of Mouse IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Ighg1

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文献 (80)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 6:75 PubMed41037185

2025

Iron accumulation in hypothalamus promotes age-dependent obesity and metabolic dysfunction of male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Wang,Xiaoyue Xiong,Ye Xuan,Wen Tian,Liwei Chen,Zhuo Chen,Yi Zhang,Wei L Shen,Cheng Hu

Current biology : CB 35:4106-4120.e7 PubMed40749677

2025

Live imaging endogenous transcription factor dynamics reveals mechanisms of epiblast and primitive endoderm fate segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca P Kim-Yip,David Denberg,Denis F Faerberg,Hayden Nunley,Isabella Leite,Madeleine Chalifoux,Bradley Joyce,Jared Toettcher,Bin Gu,Eszter Posfai

Translational psychiatry 15:126 PubMed40185739

2025

Pitolisant alleviates brain network dysfunction and cognitive deficits in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zou,Linhan Yang,Jiahui Zhu,Jihua Fan,Hanrun Zheng,Xiang Liao,Zhiqi Yang,Kuan Zhang,Hongbo Jia,Arthur Konnerth,Yan-Jiang Wang,Chunqing Zhang,Yun Zhang,Sunny C Li,Xiaowei Chen

EMBO reports 26:1792-1815 PubMed39984683

2025

Pumilio differentially binds to mRNA 3' UTR isoforms to regulate localization of synaptic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Grzejda,Anton Hess,Andrew Rezansoff,Sakshi Gorey,Judit Carrasco,Carlos Alfonso-Gonzalez,Stylianos Tsagkris,Lena Neuhaus,Mengjin Shi,Hasan Can Ozbulut,Friederike-Nora Vögtle,Andreas Vlachos,Valérie Hilgers

Advanced biology 9:e2400748 PubMed39957575

2025

KIF11 Inhibition Induces Retinopathy Progression by Affecting Photoreceptor Cell Ciliogenesis and Cell Cycle Regulation in Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xu,Jie Chen,Xin-Yao Wang,Min-Hui Huang,Xiang Wei,Xin-Rui Luo,Ya-Lan Wei,Zhen-Yu She

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406759 PubMed39840526

2025

Nuclear Condensates of WW Domain-Containing Adaptor With Coiled-Coil Regulate Mitophagy via Alternative Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahe Wang,Yi Fan,Guowen Luo,Liang Xiong,Lijie Wang,Zhuoxuan Wu,Jiayi Wang,Zhengying Peng,Clifford J Rosen,Kefeng Lu,Junjun Jing,Quan Yuan,Zhenwei Zhang,Chenchen Zhou

Redox biology 79:103469 PubMed39700693

2024

Macropinocytosis enhances foamy macrophage formation and cholesterol crystallization to activate NLRP3 inflammasome after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Zhang,Shujie Zhao,Zhenfei Huang,Ao Xue,Hao Liu,Siming Dai,Ziyang Zheng,Yin Li,Xiaodong Guo,Jun Gu,Feng Zhang,Fubing Wang,Yongxiang Wang,Xiaohua Zhou,Shujun Zhang,Hanwen Zhang,Jun Shen,Jian Chen,Guoyong Yin

Development (Cambridge, England) 151: PubMed39373366

2024

Nuclear instance segmentation and tracking for preimplantation mouse embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Hayden Nunley,Binglun Shao,David Denberg,Prateek Grover,Jaspreet Singh,Maria Avdeeva,Bradley Joyce,Rebecca Kim-Yip,Abraham Kohrman,Abhishek Biswas,Aaron Watters,Zsombor Gal,Alison Kickuth,Madeleine Chalifoux,Stanislav Y Shvartsman,Lisa M Brown,Eszter Posfai

Frontiers in neuroscience 18:1401571 PubMed39114482

2024

Retinal debris triggers cytotoxic damage in cocultivated primary porcine RPE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Wagner,Teresa Tsai,Sabrina Reinehr,Janine Theile,H Burkhard Dick,Stephanie C Joachim

Cancer innovation 3:e113 PubMed38946933

2024

Improvement of histone deacetylase inhibitor efficacy by SN38 through TWIST1 suppression in synovial sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Satoru Sasagawa,Jun Kumai,Toru Wakamatsu,Yoshihiro Yui

View all publications

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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)