Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Key features and details
- Donkey Anti-Goat IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (PE) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
特異性
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins.
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アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
吸着処理血清
Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details -
免疫原
Goat IgG whole molecule
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
特記事項（標識）
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7004の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
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| IHC-P |
Use at an assay dependent dilution.
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| IHC-Fr |
Use at an assay dependent dilution.
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| Immunomicroscopy |
Use at an assay dependent dilution.
As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications. |
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| Flow Cyt | (1) |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/250. |
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IHC-P
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
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Immunomicroscopy
Use at an assay dependent dilution. As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications. |
|
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
画像
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Dot Blot - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)
Dot Blot of ab7004.
Antigen: Goat IgG.
Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated.
Secondary antibody: ab7004 at 1:1,000 for 60 min at RT.
Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.
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Flow Cytometry - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)Verified CustomerVery bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab7004 は 9 報の論文で使用されています。
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- Kim M et al. A Recombinant Ig Fragment (IgCw-?e?) Comprising the C?1-Ce2-4 and C? Domains Is an Alternative Reagent to Human IgE. J Immunol 208:772-779 (2022). PubMed: 35022271
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- Rué L et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J Clin Invest 126:4319-4330 (2016). PubMed: 27721240
- Chen SH et al. Umbilical cord blood-derived CD34? cells improve outcomes of traumatic brain injury in rats by stimulating angiogenesis and neurogenesis. Cell Transplant 23:959-79 (2014). PubMed: 23582375
- Williams JW et al. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun 4:2990 (2013). PubMed: 24356538
- Miles A et al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008). PubMed: 18308860