Donkey Anti-Goat IgG H&L (FITC) (ab6881)

Merci de nous avoir contactés.

Vous avez du recevoir dans un autre email un devis pour l'anticorps ab6881.

J'ai reçu confirmation du laboratoire que l'anticorps ab6881 fonctionne en Western Blot. Les dilutions recommandées sont 1/5000 - 1/20000. La fiche technique a été mise à jour avec ces informations (https://www.abcam.com/ab6881).

Comme nous avons plus de 90.000 références, nous ne pouvons stocker différents volumes pour nos produits et nous n'offrons donc pas d'échantillon gratuit ou de petit volume pour des essais préliminaires.

Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique, nous offrons un remboursement ou un remplacement gratuit.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

Thank you for your protocol and your order information, we have sold only 1 vial of anti-Foxp3 to a customer in Cape town but the address is very different and it was bought in March, could this be you? I have carefully looked at each step of your protocol and would recommend the following modifications: -you say "defrost after fixing" and then mention fixing again. Can I please make sure the sections are fresh frozen and not perfusion fixed. Fresh frozen sections should be then postfixed for 20min in 4% paraformaldehyde in PB, or for 10min in ice cold acetone. -I recommend washing slides in PBS 3-4 times between each step, to decrease non specific binding -use the dilution buffer PBST to dilute the blocking agent and antibodies. PBST is made of PBS + 0.3% v/v TritonX100, to permeabilise the cells. Even if your sections are very thin they may need permeabilisation (if you fix with acetone this is not necessary as acetone fixes and permeabilises too) -block in normal serum 10% rather than BSA, this can give problems in some rare cases - try to use biotin-ABC-DAB rather than IF, as this is more specific and more sensitive. I hope these suggestions will help you, please let me know how you get on and good luck with your experiments,

Thank you for your email. For PERCP, do you mean the tandem conjugate PerCP-Cy5.5? I'm not sure what you mean by Sycron so it would great if you could provide me with some more information.