Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISA
Related conjugates and formulations
製品の概要
-
製品名
Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
アプリケーション
適用あり: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details -
吸着処理血清
Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
免疫原
Goat IgG, whole molecule
-
標識
Cy5 ®. Ex: 650nm, Em: 667nm
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
特記事項（標識）
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
-
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6566の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| WB | (1) |
Use at an assay dependent dilution.
|
| ELISA |
1/10000 - 1/50000.
|
| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
画像
-
Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).
Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.
Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).
-
Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)This image is courtesy of an anonymous AbreviewLanes 2-3 : Goat anti-UCP1 at 1/200 dilution
Lane 1 : Marker
Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg
Secondary
Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution
Observed band size: 33 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (42)
ab6566 は 42 報の論文で使用されています。
- Hennegan J et al. Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development. iScience 27:108670 (2024). PubMed: 38155767
- Li H et al. Alterations of CD8+ T cells in the blood and salivary glands of patients with primary Sjögren's syndrome. Clin Rheumatol 42:1327-1338 (2023). PubMed: 36609932
- Zhao LM et al. Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells. Bioact Mater 14:206-218 (2022). PubMed: 35310356
- Ferreira JV et al. LAMP2A regulates the loading of proteins into exosomes. Sci Adv 8:eabm1140 (2022). PubMed: 35333565
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515