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Donkey Anti-Goat IgG H&L (Biotin) preadsorbed (ab7124)

Submit a review Q&A (1)References (4)

Key features and details

  • Donkey Anti-Goat IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Donkey
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

製品の概要

  • 製品名

    Donkey Anti-Goat IgG H&L (Biotin) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Goat

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 吸着処理血清


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • 免疫原

    Full length native Goat IgG (purified).

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7124の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
特記事項
追加情報
Dot: Use at an assay dependant dilution.
ELISA: 1/16000 - 1/64000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab7124 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7124 は 4 報の論文で使用されています。

  • Kurosawa T  et al. A murine model of acute lung injury identifies growth factors to promote tissue repair and their biomarkers. Genes Cells 24:112-125 (2019). PubMed: 30474194
  • Tan S  et al. Altered Biological Properties in Dp71 Over-Expressing HBE Cells. Cell Physiol Biochem 43:2022-2036 (2017). PubMed: 29059680
  • Rienstra H  et al. Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling. Am J Transplant 9:463-72 (2009). IHC-P . PubMed: 19260830
  • Broekema M  et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. J Am Soc Nephrol 18:165-75 (2007). PubMed: 17135399

レビューと Q&A

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Question

What information do you require regarding the 3 antibodies? Due to the research we are doing, we don't want to name what the antibodies are raised to, but I can give the following information:

Ab1: mouse monoclonal IgG2A, stored in PBS (pH 7.4) and Protein A and G purified

Ab2: mouse monoclonal IgG1, stored in PBS (pH 7.4) and Protein G purified

Ab3: mouse monoclonal IgG1K, stored in PBS (pH 7.4) and Protein G purified

Is there any other information you require on the coating antibodies?

Best Wishes,

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Answer

Thank you for your message and for providing this further information.

Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so we cannot guarantee it would not crossreact with mouse. However, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation.

If you decide on a free of charge replacement, I can suggest it would be beneficial to select an alternativeantibody that has been cross absorbed with mouse, so there is minimal possible crossreactivity. For example you may like to consider the following which has been tested and guaranteed in ELISA:

ab7124 Donkey anti-Goat IgG H&L (Biotin) secondary antibody
https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html (or use the following: https://www.abcam.com/Donkey-Goat-IgG-HL-Biotin-preadsorbed-ab7124.html).

I look forward to hearing from you with details of how you would like to proceed.

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