Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 405)
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-P
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (Alexa Fluor® 405)
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
アプリケーション
適用あり: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details -
免疫原
Full length protein corresponding to Goat IgG.
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標識
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175664の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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IHC-P
Use at an assay dependent concentration. |
画像
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)
ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175664 Alexa Fluor® 405 donkey anti-goat IgG (H+L) (blue) was then used at 2µg/ml for 1h as a tertiary antibody. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab175664 は 27 報の論文で使用されています。
- Morgenstern NA et al. Pyramidal tract neurons drive amplification of excitatory inputs to striatum through cholinergic interneurons. Sci Adv 8:eabh4315 (2022). PubMed: 35138902
- Jain V et al. Gain control by sparse, ultra-slow glycinergic synapses. Cell Rep 38:110410 (2022). PubMed: 35196487
- Skoug C et al. Sphingosine 1-Phoshpate Receptors are Located in Synapses and Control Spontaneous Activity of Mouse Neurons in Culture. Neurochem Res 47:3114-3125 (2022). PubMed: 35781853
- Yanagida A et al. Naive stem cell blastocyst model captures human embryo lineage segregation. Cell Stem Cell 28:1016-1022.e4 (2021). PubMed: 33957081
- Suzuki S et al. An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia. Cell Rep 34:108752 (2021). PubMed: 33596419