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AB7125

Donkey Anti-Goat IgG H&L (HRP) preadsorbed

Donkey Anti-Goat IgG H&L (HRP) preadsorbed

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(15 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, ICC, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images
Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)
  • WB

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

* "Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons." Schaar B.T., Kinoshita K., McConnell S.K. Neuron 41 : 203213 (2004).

Minimal cortex defined : small portion of cortex removed prior to lysis.

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg

Lane 2:

Post-natal rat pup whole brain lysate (PND2-6)* at 15 µg

Lane 3:

Rat pup PND2-6 - minimal cortex brain lysate at 15 µg

Lane 4:

Rat pup PND2-6 – minimal cortex brain lysate at 35 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 15s

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)
  • WB

Unknown

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

false

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)
  • WB

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Adult mouse normal brain tissue lysate

Lane 4:

Adult rat normal brain tissue lysate

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 35 kDa,48 kDa

false

Exposure time: 15s

Key facts

宿主種

Donkey

ターゲット種

Goat

ターゲットアイソタイプ

IgG

ターゲット特異性

Heavy & Light chains

最小限の交差反応性

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

血清吸着処理済み

Yes

標識

HRP

励起波長/蛍光波長
アプリケーション

IHC-P, Dot, IM, IHC-Fr, ICC, ELISA, WB

applications

クローン性

Polyclonal

アイソタイプ

IgG

特異性

<p>No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.</p>

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組合せ製品

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB7090

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB7090)

  • 5
  • 2
Secondary Antibodies

AB97040

Goat Anti-Mouse IgG H&L (HRP) preadsorbed

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (AB97040)

  • 5
  • 2
Secondary Antibodies

AB97105

Rabbit Anti-Goat IgG H&L (HRP) preadsorbed

  • 5
  • 1
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/170000", "notes":"<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
バッファー組成
Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

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ターゲットの情報

See full target information Goat IgG
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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteomics 270:104739 PubMed36174954

2022

Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed Part II: Ileum proteomes.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Xuan Nguyen,Alessandro Agazzi,Suzanne McGill,Stefan Weidt,Quang Hanh Han,Andrea Gelemanović,Mark McLaughlin,Giovanni Savoini,Peter David Eckersall,Richard Burchmore

Molecular pharmaceutics 19:2175-2182 PubMed35642083

2022

Comparison of DLin-MC3-DMA and ALC-0315 for siRNA Delivery to Hepatocytes and Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Ferraresso,Amy W Strilchuk,Lih Jiin Juang,Lauren G Poole,James P Luyendyk,Christian J Kastrup

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Molecular medicine reports 23: PubMed33537819

2021

CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia‑induced damage.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Liu,Hongchao Zhao,Qingqing Ding,Haohao Li,Tao Liu,Hongwei Yang,Yuanhua Liu

Experimental and therapeutic medicine 20:854-859 PubMed32742328

2020

Ameliorating effect of quercetin on epilepsy by inhibition of inflammation in glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Wu,Zihui Zheng,Shaohua Fan,Xin Wen,Xinrui Han,Shan Wang,Yongjian Wang,Zifeng Zhang,Qun Shan,Mengqiu Li,Bin Hu,Yuanlin Zheng,Jun Lu

Experimental and therapeutic medicine 18:4313-4321 PubMed31777538

2019

Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Luyifei Li,Ling Jing,Jiyi Zhao,Jiachen Lv,Wen Yang,Weimin Li,Lijun Zhou

Oncology 97:26-37 PubMed31071716

2019

Tropomyosin-Related Kinase Receptor and Neurotrophin Expression in Cutaneous Melanoma Is Associated with a Poor Prognosis and Decreased Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Luís Carlos Moreira Antunes,André Cartell,Caroline Brunetto de Farias,Renato Marchiori Bakos,Rafael Roesler,Gilberto Schwartsmann

Cell death & disease 8:e2842 PubMed28569781

2017

Mitochondrial AKAP1 supports mTOR pathway and tumor growth.

Applications

IHC

Species

Unspecified reactive species

Laura Rinaldi,Maria Sepe,Rossella Delle Donne,Kristel Conte,Antonietta Arcella,Domenica Borzacchiello,Stefano Amente,Fernanda De Vita,Monia Porpora,Corrado Garbi,Maria A Oliva,Claudio Procaccini,Deriggio Faicchia,Giuseppe Matarese,Federica Zito Marino,Gaetano Rocco,Sara Pignatiello,Renato Franco,Luigi Insabato,Barbara Majello,Antonio Feliciello

Journal of neuroinflammation 14:59 PubMed28320424

2017

The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Jendresen,Vibeke Årskog,Michael R Daws,Lars N G Nilsson

Proceedings of the National Academy of Sciences of 114:E337-E346 PubMed28049839

2017

GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Nassar,Yaara Tabib,Tal Capucha,Gabriel Mizraji,Tsipora Nir,Meirav Pevsner-Fischer,Gili Zilberman-Schapira,Oded Heyman,Gabriel Nussbaum,Herve Bercovier,Asaf Wilensky,Eran Elinav,Tal Burstyn-Cohen,Avi-Hai Hovav
View all publications
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