Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
Key features and details
- Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
- Host species: Donkey
- Suitable for: IHC-P, IHC-Fr, ELISA, ICC/IF, Flow Cyt
製品の概要
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製品名
Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
特異性
Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins. -
アプリケーション
適用あり: IHC-P, IHC-Fr, ELISA, ICC/IF, Flow Cytmore details -
吸着処理血清
Human, Mouse, Rat more details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 568. Ex: 578nm, Em: 603nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Gel filtration chromatography of pepsin digested antibody -
ポリ/モノ
ポリクローナル -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175694の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| Flow Cyt |
1/2000.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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Flow Cyt
1/2000. |
画像
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Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175694 Alexa Fluor® 568 donkey F(ab')2 anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
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Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175694 Alexa Fluor® 568 donkey F(ab')2 anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.Alexa Fluor® 568 is pseudocolored in yellow.
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Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab175694 は 21 報の論文で使用されています。
- Kim J et al. Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells. Nat Protoc 17:890-909 (2022). PubMed: 35140411
- Liskiewicz D et al. Differential Response of Hippocampal and Cerebrocortical Autophagy and Ketone Body Metabolism to the Ketogenic Diet. Front Cell Neurosci 15:733607 (2021). PubMed: 34456688
- Dave K et al. Increased expression of PD-1 and PD-L1 in oral lesions progressing to oral squamous cell carcinoma: a pilot study. Sci Rep 10:9705 (2020). PubMed: 32546692
- Mladineo I & Hrabar J Leukocyte Nucleolus and Anisakis pegreffii-When Falling Apart Means Falling in Place. Genes (Basel) 11:N/A (2020). PubMed: 32585969
- Janssen A et al. ?d T-cell Receptors Derived from Breast Cancer-Infiltrating T Lymphocytes Mediate Antitumor Reactivity. Cancer Immunol Res 8:530-543 (2020). PubMed: 32019779