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AB325285

Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488)

Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488)

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Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488) (ab325285) is a ready-to-go fluorescent camelid single-domain antibody (sdAb,nanobody, VHH) conjugate with maximum absorption wavelength of 499 nm and a maximum emission wavelength of 520 nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF applications.

  • Quantitative and reliable readout in fluorescence-based analyses
  • Defined number of fluorophores enhance brightness of signals
  • Improved localization accuracy ideal for super resolution imaging
  • Designed to recognize conventional immunoglobulins with high isotype specificity

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images
Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488) (AB325285)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488) (AB325285)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilized, quenched with 0.1M ammonium chloride, and blocked with 10% normal goat serum in PBS HeLa (human cervical cancer cells) cells labelling Vimentin with ab316214 at 1/1000 (0.80 µg/ml) dilution, followed by ab325285 Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488) at 1/500 (0.15 µg/ml per sdAb) dilution (green).

Confocal image showing cytoplasmic staining in HeLa cells (shown in green). The counterstain was observed in purple. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a fluorescence microscope (Zeiss Axio Imager Z1).

ab92742 anti-Ki67 antibody was used to counterstain Ki67 at 1/1000 (0.85 µg/ml) dilution (purple).

Secondary antibody only control : Secondary antibody is ab325285 Alpaca monoclonal [3G2] Anti-Chicken IgY antibody (ATTO 488) at 1/500 (0.15 µg/ml per sdAb) dilution.

Key facts

宿主種

Alpaca

ターゲット種

Chicken

ターゲットアイソタイプ

IgY

ターゲット特異性

Heavy & Light chains

最小限の交差反応性
血清吸着処理済み

No

標識

ATTO 488

励起波長/蛍光波長

Ex: 501nm, Em: 523nm

アプリケーション

ICC/IF

applications

クローン性

Monoclonal

クローン番号

3G2

アイソタイプ

IgG

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":"<p></p>" } } }

製品の詳細

Together with their monovalent binding mode, the defined fluorophore conjugation of ab325285 results in a quantitative and reliable readout for several fluorescence-based applications.  It contains a defined number of fluorophores at known positions. In addition due to the small size the fluorophore displacement from the bound target protein is very low making ab325285 ideal for standard super resolution technologies.

Features:
- High affinity monovalent binding + two fluorophores per sdAb for brighter signals.
- Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
In comparison to conventional secondary reagents the ab325285 nanobody has the following advantages:
- Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
- Monoclonal tools with defined species  and isotype specificity.
- Recombinant production for reliable quality, scalability and reproducibility.
- Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible.
- Enhanced binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing).
- Suitable for post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.).

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
バッファー組成
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1 month
短期保存温度
-20°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

ターゲットの情報

See full target information Chicken IgY
websiteProtocolBooklet
en

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