Whole Cell Extraction Kit (ab113475)
Key features and details
- Assay time: 45 min
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Whole Cell Extraction Kit -
サンプルの種類
Adherent cells, Suspension cells -
全工程の試験時間
0h 45m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals
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製品の概要
Abcam's Whole Cell Extraction Kit (ab113475) provides a simple and selective method for extracting whole cell proteins in just 45 minutes for a variety of applications such as western blotting, cellular enzyme assays, or any other procedures requiring cellular proteins. ab113475 can be used to extract cellular proteins from mammalian cells and tissue samples.
Not sure if this is the right product for you? Check out our Methods and tools to study histone modifications for help.
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 100 tests 1000X DTT Solution 1 x 100µl 1000X Protease Inhibitor Cocktail 1 x 100µl 5X Extraction Buffer 1 x 20ml -
研究分野
画像
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Functional Studies - Whole Cell Extraction Kit (ab113475)Whole cell extracts were prepared from MCF-7 cells using ab113475. Activity of SOD was measured using different amounts of the extract.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab113475 は 6 報の論文で使用されています。
- Ji QX et al. Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis. Cell Death Differ 30:457-474 (2023). PubMed: 36477078
- Zhu B & McBride JW Alpha Enolase 1 Ubiquitination and Degradation Mediated by Ehrlichia chaffeensis TRP120 Disrupts Glycolytic Flux and Promotes Infection. Pathogens 10:N/A (2021). PubMed: 34451426
- Al-Dwairi A et al. Metformin exerts anti-inflammatory effects on mouse colon smooth muscle cells in vitro. Exp Ther Med 16:985-992 (2018). PubMed: 30116349
- Hannibal L et al. Transcellular transport of cobalamin in aortic endothelial cells. FASEB J 32:5506-5519 (2018). PubMed: 29741927
- Zhu B et al. Rapid identification of ubiquitination and SUMOylation target sites by microfluidic peptide array. Biochem Biophys Rep 5:430-438 (2016). PubMed: 27047992
- Peng YT et al. Upregulation of cyclin-dependent kinase inhibitors CDKN1B and CDKN1C in hepatocellular carcinoma-derived cells via goniothalamin-mediated protein stabilization and epigenetic modifications. Toxicol Rep 2:322-332 (2015). PubMed: 28962365