AB65400

Plasma Membrane Protein Extraction Kit

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(203 Publications)

Plasma membrane protein extraction kit (ab65400) provides optimized buffers and reagents for the effective extraction of native plasma membrane proteins from mammalian tissues and cells. Suitable for downstream applications such as western bot and SDS-PAGE.

- Option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins
- Compatible with our Lab essentials western blot workflow offering
- Proven performance: cited in over 200 publications

2 Images

PubMed

Western blot - Plasma Membrane Protein Extraction Kit (AB65400)

Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.

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Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400)

FuncS

Unknown

Functional Studies - Plasma Membrane Protein Extraction Kit (AB65400)

Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.

Kweon, Hae-Jin et al., Scientific reports: vol. 6 30684., Fig 1, doi:10.1038/srep30684

Key facts

アッセイ時間

サンプルタイプ

Tissue Homogenate, Cell culture extracts

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製品の詳細

Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.

Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.

The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

This product is manufactured by BioVision, an Abcam company and was previously called K268 Membrane Protein Extraction Kit. K268-50 is the same size as the 50 test size of ab65400.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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製品内容

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab65400-50Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Upper Phase Solution", "number":"AB65400-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Lower Phase Solution", "number":"AB65400-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB65400-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"Homogenization Buffer I", "number":"AB65400-CMP02", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

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文献 (203)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 21:7 PubMed38178138

2024

The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect.

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Bo-Kyoung Jung,Yong Hee An,Sung Hoon Jang,Jin-Ju Jang,Seonhee Kim,Joo Hee Jeon,Jinju Kim,Jason Jungsik Song,Hyun Jang

Cell death & disease 14:761 PubMed37996408

2023

Targeting protein methylation in pancreatic cancer cells results in KRAS signaling imbalance and inhibition of autophagy.

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María F Montenegro,Román Martí-Díaz,Ana Navarro,Jorge Tolivia,Luis Sánchez-Del-Campo,Juan Cabezas-Herrera,José Neptuno Rodríguez-López

Nature communications 14:4989 PubMed37591837

2023

Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscle.

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Anastasia Sacharidou,Ken Chambliss,Jun Peng,Jose Barrera,Keiji Tanigaki,Katherine Luby-Phelps,İpek Özdemir,Sohaib Khan,Shashank R Sirsi,Sung Hoon Kim,Benita S Katzenellenbogen,John A Katzenellenbogen,Mohammed Kanchwala,Adwait A Sathe,Andrew Lemoff,Chao Xing,Kenneth Hoyt,Chieko Mineo,Philip W Shaul

Phytotherapy research : PTR 37:3898-3912 PubMed37132081

2023

Sclareol attenuates liver fibrosis through SENP1-mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling.

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Mao-Xu Ge,Wei-Xiao Niu,Yun-Yang Bao,Zhen-Ning Lu,Hong-Wei He

Cancer research 83:2034-2051 PubMed37078777

2023

ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma.

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Yan Sun,Liang Zhu,Pian Liu,Huan Zhang,Feng Guo,Xin Jin

Nature communications 14:1785 PubMed36997581

2023

Defective BVES-mediated feedback control of cAMP in muscular dystrophy.

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Haiwen Li,Peipei Wang,Chen Zhang,Yuanbojiao Zuo,Yuan Zhou,Renzhi Han

Journal of periodontal research 58:634-645 PubMed36919895

2023

Pre-conditioning of gingival epithelial cells with sub-apoptotic concentrations of curcumin prevents pro-inflammatory cytokine release.

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Melissa M Grant,Ann E Scott,John B Matthews,Helen R Griffiths,Iain L C Chapple

Nature communications 14:870 PubMed36797266

2023

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

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Karsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian Behrends

Frontiers in immunology 14:1090202 PubMed36798132

2023

Inhibition of sphingosine-1-phosphate receptor 3 suppresses ATP-induced NLRP3 inflammasome activation in macrophages TWIK2-mediated potassium efflux.

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Yingqin Wang,Chen Wang,Qiaolan He,Guannan Chen,Jie Yu,Jing Cang,Ming Zhong

Science translational medicine 14:eabj4221 PubMed36542691

2022

A monoclonal antibody targeting nonjunctional claudin-1 inhibits fibrosis in patient-derived models by modulating cell plasticity.

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Species

Unspecified reactive species

Natascha Roehlen,Antonio Saviano,Houssein El Saghire,Emilie Crouchet,Zeina Nehme,Fabio Del Zompo,Frank Jühling,Marine A Oudot,Sarah C Durand,François H T Duong,Sara Cherradi,Victor Gonzalez Motos,Nuno Almeida,Clara Ponsolles,Laura Heydmann,Tessa Ostyn,Antonin Lallement,Patrick Pessaux,Emanuele Felli,Andrea Cavalli,Jacopo Sgrignani,Christine Thumann,Olga Koutsopoulos,Bryan C Fuchs,Yujin Hoshida,Maike Hofmann,Mogens Vyberg,Birgitte Martine Viuff,Elisabeth D Galsgaard,Greg Elson,Alberto Toso,Markus Meyer,Roberto Iacone,Tamas Schweighoffer,Geoffrey Teixeira,Solange Moll,Claudio De Vito,Tania Roskams,Irwin Davidson,Danijela Heide,Mathias Heikenwälder,Mirjam B Zeisel,Joachim Lupberger,Laurent Mailly,Catherine Schuster,Thomas F Baumert

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Plasma Membrane Protein Extraction Kit