Nuclear Extraction Kit (ab113474)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
製品の概要
-
製品名
Nuclear Extraction Kit
Nuclear Extraction キット 製品一覧 -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
全工程の試験時間
1h 00m -
製品の概要
Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for nuclear protein extraction / nuclear protein fractionation in just 1 hour.
The extracts can then be used in western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
Not sure if this is the right product for you? Check out our Methods and tools to study histone modifications for help.
Compared to other kits that use conventional nuclear extraction / nuclear fractionation methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which allows much better retention of enzyme activity in the nuclear extracts.
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Pre-Extraction Buffer 1 x 10ml DTT Solution (1000X) 1 x 110µl ENE2 (Extraction Buffer) 1 x 10ml -
研究分野
関連製品
-
Related Products
画像
-
Functional Studies - Nuclear Extraction Kit (ab113474)Ragab, Diaa et al., PloS one?vol. 9,5 e95313., Fig 6, doi:10.1371/journal.pone.0095313
Effect of two drugs refered to as Cilo (50 and 100 mg/kg; Cilo50and Cilo100), and Pio (3 and 10 mg/kg; Pio3 and Pio10), and their combination (Cilo50and Pio3) on the PPAR-γ transcription activity in rats subjected to ischemia (45 min)/reperfusion (24 hrs).
Drugs were administered orally for 14 days then subjected to ischemia/reperfusion. Values are expressed as mean ± S.E.M (n = 6). Data are compared with sham operated control (#), I/R control (∗), Cilo50 (), Pio3 (), and combination (§) pretreated groups (one-way ANOVA followed by Tukey Multiple Comparison Test) at P<0.05. -
Functional Studies - Nuclear Extraction Kit (ab113474)Park, Jisu et al., PloS one?vol. 10,11 e0141988., Fig 4, doi:10.1371/journal.pone.0141988B16F10 cells were treated with 30 µM of DMPB for the indicated time periods. Cytoplasmic and nuclear fractions were isolated and analyzed by Western blotting. -
Functional Studies - Nuclear Extraction Kit (ab113474)Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (256)
ab113474 は 256 報の論文で使用されています。
- Zhu JQ et al. Modified Qing-Zao-Jiu-Fei decoction attenuated pulmonary fibrosis induced by bleomycin in rats via modulating Nrf2/NF-κB and MAPKs pathways. Chin Med 19:10 (2024). PubMed: 38229198
- Liu W et al. Blockade of IL-6 signaling alleviates atherosclerosis in Tet2-deficient clonal hematopoiesis. Nat Cardiovasc Res 2:572-586 (2023). PubMed: 37539077
- Han K et al. Boosting NAD preferentially blunts Th17 inflammation via arginine biosynthesis and redox control in healthy and psoriasis subjects. Cell Rep Med 4:101157 (2023). PubMed: 37586364
- Zhu W et al. Fructose-1,6-bisphosphatase 1 dephosphorylates IκBα and suppresses colorectal tumorigenesis. Cell Res 33:245-257 (2023). PubMed: 36646759
- Wuestefeld A et al. A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes. Cancer Res 83:428-440 (2023). PubMed: 36449018