AB113474

Nuclear Extraction Kit

Name: Nuclear Extraction Kit (ab113474) | Abcam
Brand: Abcam Limited
SKU: AB113474
Rating: 5 (3 reviews)

(3 Reviews)

(296 Publications)

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications.

- Cited in over 255 publications
- High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention
- Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more
- Fast, simple procedure that ensures optimized nuclear protein isolation

3 Images

Functional Studies - Nuclear Extraction Kit (AB113474)

FuncS

Supplier Data

Functional Studies - Nuclear Extraction Kit (AB113474)

Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.

FuncS

Unknown

Functional Studies - Nuclear Extraction Kit (AB113474)

Effect of two drugs refered to as Cilo (50 and 100 mg/kg; Cilo50and Cilo100), and Pio (3 and 10 mg/kg; Pio3 and Pio10), and their combination (Cilo50and Pio3) on the PPAR-γ transcription activity in rats subjected to ischemia (45 min)/reperfusion (24 hrs).
Drugs were administered orally for 14 days then subjected to ischemia/reperfusion. Values are expressed as mean ± S.E.M (n = 6). Data are compared with sham operated control (#), I/R control (∗), Cilo50 (), Pio3 (), and combination (§) pretreated groups (one-way ANOVA followed by Tukey Multiple Comparison Test) at P<0.05.

Ragab, Diaa et al., PloS one: vol. 9,5 e95313., Fig 6, doi:10.1371/journal.pone.0095313

FuncS

Unknown

Functional Studies - Nuclear Extraction Kit (AB113474)

B16F10 cells were treated with 30 µM of DMPB for the indicated time periods. Cytoplasmic and nuclear fractions were isolated and analyzed by Western blotting.

Park, Jisu et al., PloS one: vol. 10,11 e0141988., Fig 4, doi:10.1371/journal.pone.0141988

Key facts

アッセイ時間

サンプルタイプ

Tissue, Suspension cells, Adherent cells

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製品の詳細

Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for nuclear protein extraction / nuclear protein fractionation in just 1 hour.

The extracts can then be used in western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.

Not sure if this is the right product for you? Check out our Methods and tools to study histone modifications for help.

Compared to other kits that use conventional nuclear extraction / nuclear fractionation methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which allows much better retention of enzyme activity in the nuclear extracts.

The 1M DTT included in this kit may precipitate if exposed to temperatures below the recommended storage range. While the kit should be stored at 4°C, temperatures lower than this -such as those sometimes found in lab refrigerators- can lead to precipitation.
If precipitation occurs, we recommend re-dissolving the DTT by gently shaking the vial in a 37°C water bath until fully dissolved.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

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文献 (296)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1546010 PubMed40606616

2025

Innovative pH-responsive alginate-coated rosuvastatin-loaded chitosan nanoparticles: a targeted approach to inhibiting HMGB1-activated RAGE/TLR4-NFκB signaling in colonic inflammation in rats.

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Elsayed A Elmorsy,Sameh Saber,Zubida M Al-Majdoub,Rabab S Hamad,Mustafa Ahmed Abdel-Reheim,Asmaa Ramadan,Norah Suliman Alsoqih,Mariam S Alharbi,Hamad Alsaykhan,Alshaimaa A Farrag,Hanan Eissa,Rasha Abdelhady,Abousree T Ellethy,Mostafa M Khodeir,Hossam A Elsisi,Syed Suhail Ahmed,Ahmed Y Kira

Translational psychiatry 15:198 PubMed40506434

2025

Concentration-dependent effect of delta-9-tetrahydrocannabinol on epigenetic DNA modifiers in human peripheral blood mononuclear cells.

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Kerda Pulk,Kelli Somelar-Duracz,Mikk Rooden,Kaili Anier,Anti Kalda

Current protocols 5:e70143 PubMed40498292

2025

Epigenetic Histone Deacetylases Activity Assay in the Brain and Peripheral Organ Tissues.

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Doodipala Samba Reddy,Hudson Boyd,Vinitha Karnati,Joon Kim,Sreevidhya Ramakrishnan,Xin Wu

Veterinary sciences 12: PubMed40431525

2025

Comparative Evaluation of Takhrai () Leaf Extracts with Commercial Antioxidants for Oxidative Stress Mitigation in Ruminants Under Heat Stress.

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Rayudika Aprilia Patindra Purba,Phanthipha Laosam,Nattapol Pongsamai,Papungkorn Sangsawad

Biomolecules 15: PubMed40427627

2025

Uncovering a Novel Role of ROR1 in the Epigenetic Regulation of Tumor Suppressor Gene CREB3L1 in Triple-Negative Breast Cancer Cells.

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Victoria L Reed,Eric Lalu,Leena Yoon,Norman Fultang,Bela Peethambaran

Molecular medicine reports 32: PubMed40280116

2025

Understanding the role of iron/heme metabolism in the anti‑inflammatory effects of natural sulfur molecules against lipopolysaccharide‑induced inflammation.

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Dong Young Kang,Se Won Bae,Kyoung-Jin Jang

American journal of cancer research 15:1133-1147 PubMed40226449

2025

Itaconate targets the ERK2 signal to suppress estrogen receptor-positive breast cancer cell growth.

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Hsueh-Chun Wang,Yi-Chuan Li,Mien-Chie Hung

Scientific reports 15:12516 PubMed40216954

2025

CaMK2A/CREB pathway activation is associated with enhanced mitophagy and neuronal apoptosis in diabetic retinopathy.

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Xiaochun Yang,Yuxin Zhang,Yikun Zhou,Mingzhi Liu,Haiyan Zhao,Yang Yang,Jianyun Su

Molecular biology reports 52:322 PubMed40100290

2025

Skeletal muscle atrophy induced by aging and disuse atrophy are strongly associated with the upregulation of the endoplasmic stress protein CHOP in rats.

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J Max Michel,Joshua S Godwin,Nathan R Kerr,Thomas E Childs,Frank W Booth,C Brooks Mobley,David C Hughes,Michael D Roberts

Foods (Basel, Switzerland) 14: PubMed40077496

2025

Effects of Dietary Pretreatment with All- Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon.

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Daolin Mou,Dajiang Ding,Junning Pu,Pan Zhou,Enming Cao,Xueyan Zhang,Junrong Lan,Lu Ye,Wanxue Wen

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Nuclear Extraction Kit