AB110168

Mitochondria Isolation Kit for Tissue

Name: Mitochondria Isolation Kit for Tissue (ab110168) | Abcam
Brand: Abcam Limited
SKU: AB110168
Rating: 5 (2 reviews)

(2 Reviews)

(67 Publications)

Mitochondria Isolation Kit for Tissue (ab110168) is designed for efficient isolation of mitochondria from mammalian tissue using differential centrifugation. Suitable for Western blot, immunocapture or activity assays.

- Provides reagents for up to 10 isolations
- Proven performance: cited in over 65 publications

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Supplier Data

Western blot - Mitochondria Isolation Kit for Tissue (AB110168)

Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 μg (lane 1) and 10 μg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.

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Key facts

アッセイ時間

サンプルタイプ

Tissue

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製品の詳細

ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze

Tested tissues: brain, liver and heart

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab110168-1Kit", "assetComponentDetails": [ { "size":"1 x 100 mL", "name":"Isolation Buffer", "number":"AB110168-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Wash Buffer", "number":"AB110168-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

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文献 (67)

Recent publications for all applications. Explore the full list and refine your search

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 31:101817 PubMed37915829

2023

Protective effect of eriodictyol against hyperglycemia-induced diabetic nephropathy in rats entails antioxidant and anti-inflammatory effects mediated by activating Nrf2.

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Unspecified reactive species

Jozaa Z AlTamimi,Nora A AlFaris,Ghedeir M Alshammari,Reham I Alagal,Dalal H Aljabryn,Mohammed Abdo Yahya

Nature communications 14:5351 PubMed37660128

2023

Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish.

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Wenwei Sun,Meimei Wang,Jun Zhao,Shuang Zhao,Wenchao Zhu,Xiaoting Wu,Feifei Li,Wei Liu,Zhuo Wang,Meng Gao,Yiyue Zhang,Jin Xu,Meijia Zhang,Qiang Wang,Zilong Wen,Juan Shen,Wenqing Zhang,Zhibin Huang

JACC. Basic to translational science 8:1215-1239 PubMed37791317

2023

Syntaxin 17 Protects Against Heart Failure Through Recruitment of CDK1 to Promote DRP1-Dependent Mitophagy.

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Haixia Xu,Xiang Wang,Wenjun Yu,Shiqun Sun,Ne N Wu,Junbo Ge,Jun Ren,Yingmei Zhang

Disease models & mechanisms 16: PubMed37260288

2023

NR2F1 shapes mitochondria in the mouse brain, providing new insights into Bosch-Boonstra-Schaaf optic atrophy syndrome.

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Sara Bonzano,Eleonora Dallorto,Ivan Molineris,Filippo Michelon,Isabella Crisci,Giovanna Gambarotta,Francesco Neri,Salvatore Oliviero,Ruth Beckervordersandforth,Dieter Chichung Lie,Paolo Peretto,Serena Bovetti,Michèle Studer,Silvia De Marchis

Nature 616:152-158 PubMed36991121

2023

Norovirus MLKL-like protein initiates cell death to induce viral egress.

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Guoxun Wang,Di Zhang,Robert C Orchard,Dustin C Hancks,Tiffany A Reese

Nutrients 15: PubMed37049501

2023

The Protective Effect of 11-Keto-β-Boswellic Acid against Diabetic Cardiomyopathy in Rats Entails Activation of AMPK.

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Jozaa Z AlTamimi,Nora A AlFaris,Ghedeir M Alshammari,Reham I Alagal,Dalal H Aljabryn,Mohammed Abdo Yahya

The European journal of neuroscience 57:1018-1032 PubMed36750311

2023

Decreased β-hydroxybutyrate and ketogenic amino acid levels in depressed human adults.

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Shiho Sato,Zhiqian Yu,Mai Sakai,Ikuko N Motoike,Daisuke Saigusa,Ryo Hirayama,Yoshie Kikuchi,Takaaki Abe,Kengo Kinoshita,Seizo Koshiba,Hiroaki Tomita

Neurobiology of stress 21:100499 PubMed36532369

2022

Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF.

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Alzahra J Al Omran,Saki Watanabe,Ethan C Hong,Samantha G Skinner,Mindy Zhang,Jifeng Zhang,Xuesi M Shao,Jing Liang

Turkish journal of medical sciences 52:1468-1477 PubMed36422496

2022

Impact of ATP synthase/coupling factor 6 in hypoxic pulmonary arterial hypertension: An experimental rat model.

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Nannan Lı,Yugen Shı,Jie Yın,Li Sun,Qingshan Zhang,Shuai Bao,Juan Zhang,Youlei Lı,Miaomiao Wang,Yanwei Zhang,Mei Xue,Lei Qı,Yan Lı,Suhua Yan,Xiaolu Lı

Cell 185:4216-4232.e16 PubMed36240780

2022

En masse organoid phenotyping informs metabolic-associated genetic susceptibility to NASH.

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Masaki Kimura,Takuma Iguchi,Kentaro Iwasawa,Andrew Dunn,Wendy L Thompson,Yosuke Yoneyama,Praneet Chaturvedi,Aaron M Zorn,Michelle Wintzinger,Mattia Quattrocelli,Miki Watanabe-Chailland,Gaohui Zhu,Masanobu Fujimoto,Meenasri Kumbaji,Asuka Kodaka,Yevgeniy Gindin,Chuhan Chung,Robert P Myers,G Mani Subramanian,Vivian Hwa,Takanori Takebe

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Mitochondria Isolation Kit for Tissue