AB110168

Mitochondria Isolation Kit for Tissue

Name: Mitochondria Isolation Kit for Tissue (ab110168) | Abcam
Brand: Abcam Limited
SKU: AB110168
Price: 41000 JPY
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(80 Publications)

Mitochondria Isolation Kit for Tissue (ab110168) is designed for efficient isolation of mitochondria from mammalian tissue using differential centrifugation. Suitable for Western blot, immunocapture or activity assays.

- Provides reagents for up to 10 isolations
- Proven performance: cited in over 75 publications

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Supplier Data

Western blot - Mitochondria Isolation Kit for Tissue (AB110168)

Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 μg (lane 1) and 10 μg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.

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Key facts

アッセイ時間

サンプルタイプ

Tissue

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製品の詳細

ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze

Tested tissues: brain, liver and heart

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab110168-1Kit", "assetComponentDetails": [ { "size":"1 x 100 mL", "name":"Isolation Buffer", "number":"AB110168-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Wash Buffer", "number":"AB110168-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

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文献 (80)

Recent publications for all applications. Explore the full list and refine your search

The AAPS journal 27:112 PubMed40562976

2025

Pharmacokinetics and Pharmacodynamics of Nomlabofusp in Non-clinical Studies of Friedreich's Ataxia.

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Flavia De Toni,Vanessa Ragaglia,Devin Schecter,Angela S Miller,Eric Gonzalez,Erik J Wagner,Xin Xu,R Mark Payne,Jean-Nicholas Mess,Matthew G Baile,Adrienne Clements-Egan,Gopi Shankar

Investigational new drugs 43:669-678 PubMed40404898

2025

Peptide YY fragment PYY1-36 disrupts mitochondrial biogenesis via RBM43-dependent PGC-1α translation inhibition.

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Benkun Liu,Fucheng Zhou,Bowen Shi,Yubo Yan,Yanbo Wang,Junfeng Wang,Yaoguo Lang,Shidong Xu

Communications biology 8:722 PubMed40346382

2025

Krüppel like factor 7 regulates mitochondrial dynamics balance in myocardial infarction.

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Cao Wang,Fuxing Zhu,Lan Zhou,Situo Zhang,Ruiqi Wang,Hui Tian,Bosong Zhang,Jiahui Wu,Xiang Xu,Ruixian Jiang,Xiaolu Hou,Jian Liu,Weiming Tian

Antioxidants (Basel, Switzerland) 13: PubMed39594567

2024

25-Hydroxycholecalciferol Improves Cardiac Metabolic Adaption, Mitochondrial Biogenetics, and Redox Status to Ameliorate Pathological Remodeling and Functional Failure in Obese Chickens.

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Shih-Kai Chiang,Mei-Ying Sin,Jun-Wen Lin,Maraddin Siregar,Gilmour Valdez,Yu-Hui Chen,Thau Kiong Chung,Rosemary L Walzem,Lin-Chu Chang,Shuen-Ei Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404391 PubMed39444078

2024

Membrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice.

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Zhongqiu Zhou,Ying Bai,Xiaochun Gu,Hui Ren,Wen Xi,Yu Wang,Liang Bian,Xue Liu,Ling Shen,Xianyuan Xiang,Wenhui Huang,Zhuojuan Luo,Bing Han,Honghong Yao

Molecular metabolism 88:102018 PubMed39182844

2024

Glucose-1,6-bisphosphate: A new gatekeeper of cerebral mitochondrial pyruvate uptake.

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Motahareh Solina Safari,Priska Woerl,Carolin Garmsiri,Dido Weber,Marcel Kwiatkowski,Madlen Hotze,Louisa Kuenkel,Luisa Lang,Matthias Erlacher,Ellen Gelpi,Johannes A Hainfellner,Gottfried Baier,Gabriele Baier-Bitterlich,Stephanie Zur Nedden

mBio 15:e0129224 PubMed38860823

2024

Skeletal muscle mitochondrial dysfunction mediated by quorum-sensing transcription factor MvfR: reversing effects with anti-MvfR and mitochondrial-targeted compounds.

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Shifu Aggarwal,Vijay Singh,Arijit Chakraborty,Sujin Cha,Alexandra Dimitriou,Claire de Crescenzo,Olivia Izikson,Lucy Yu,Roberto Plebani,A Aria Tzika,Laurence G Rahme

Clinical and translational medicine 14:e1680 PubMed38769668

2024

Targeting c-Myc transactivation by LMNA inhibits tRNA processing essential for malate-aspartate shuttle and tumour progression.

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Jianqun Wang,Mei Hong,Yang Cheng,Xiaojing Wang,Dan Li,Guo Chen,Banghe Bao,Jiyu Song,Xinyi Du,Chunhui Yang,Liduan Zheng,Qiangsong Tong

Cell reports 43:114237 PubMed38753484

2024

Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia.

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Yan Tang,Junru Wu,Xuejing Sun,Shasha Tan,Wenbo Li,Siyu Yin,Lun Liu,Yuanyuan Chen,Yuanyuan Liu,Qian Tan,Youxiang Jiang,Wenjing Yang,Wei Huang,Chunyan Weng,Qing Wu,Yao Lu,Hong Yuan,Qingzhong Xiao,Alex F Chen,Qingbo Xu,Timothy R Billiar,Jingjing Cai

Lipids in health and disease 23:68 PubMed38431645

2024

The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury.

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Yichang Liu,Zhaoling Sun,Qiuli Sun,Li Wang,Chuan Wang,Yingmin Li,Chunling Ma,Weibo Shi,Guozhong Zhang,Yiming Dong,Xiaojing Zhang,Bin Cong

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mitochondria Isolation Kit for Tissue