AB110170

Mitochondria Isolation Kit for Cultured Cells

Name: Mitochondria Isolation Kit for Cultured Cells (ab110170) | Abcam
Brand: Abcam Limited
SKU: AB110170
Rating: 5 (2 reviews)

(2 Reviews)

(79 Publications)

Mitochondria isolation kit for cultured cells (ab110170) is designed for efficient isolation of mitochondria from cultured mammalian cells using differential centrifugation. Suitable for Western blot, immunocapture or activity assays.

- Provides reagents for up to 20 isolations
- Proven performance: cited in over 75 publications

1 Images

Supplier Data

Western blot - Mitochondria Isolation Kit for Cultured Cells (AB110170)

Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

false

Key facts

アッセイ時間

交差種

Mammals

style

left-column

製品の詳細

ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Available with a dounce homogenizer as kit ab110171.

Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze

style

left-column

製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab110170-1Kit", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Reagent C", "number":"AB110170-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent B", "number":"AB110170-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent A", "number":"AB110170-CMP01", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

style

left-column

文献 (79)

Recent publications for all applications. Explore the full list and refine your search

Journal of respiratory biology and translational medicine 1: PubMed38737375

2024

The Asthma Risk Gene, , Promotes Mitochondrial DNA-induced Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Julian Hecker,Siqi Liu,Xianliang Rui,Nathan Boyer,Jennifer Wang,Yuzhen Yu,Yihan Zhang,Hongmei Mou,Luis Guillermo Gomez-Escobar,Augustine M K Choi,Benjamin A Raby,Scott T Weiss,Xiaobo Zhou

Glia 72:184-205 PubMed37668005

2023

ApoE4 expression disrupts tau uptake, trafficking, and clearance in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maxwell Eisenbaum,Andrew Pearson,Camila Ortiz,Michael Mullan,Fiona Crawford,Joseph Ojo,Corbin Bachmeier

iScience 26:107558 PubMed37664623

2023

-encoded microprotein mitoregulin regulates fatty acid metabolism at the mitochondrial outer membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Zhang,Yabo Guo,Gio Fidelito,David R L Robinson,Chao Liang,Radiance Lim,Zoë Bichler,Ruiyang Guo,Gaoqi Wu,He Xu,Quan D Zhou,Brijesh K Singh,Paul Yen,Dennis Kappei,David A Stroud,Lena Ho

JACC. Basic to translational science 8:1215-1239 PubMed37791317

2023

Syntaxin 17 Protects Against Heart Failure Through Recruitment of CDK1 to Promote DRP1-Dependent Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Xu,Xiang Wang,Wenjun Yu,Shiqun Sun,Ne N Wu,Junbo Ge,Jun Ren,Yingmei Zhang

Journal of cell science 136: PubMed37303235

2023

Aggregation-prone Tau impairs mitochondrial import, which affects organelle morphology and neuronal complexity.

Applications

Unspecified application

Species

Unspecified reactive species

Hope I Needs,Kevin A Wilkinson,Jeremy M Henley,Ian Collinson

Journal of neuroinflammation 20:113 PubMed37170230

2023

β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium.

Applications

Unspecified application

Species

Unspecified reactive species

Fuzhou Hua,Hong Zhu,Wen Yu,Qingcui Zheng,Lieliang Zhang,Weidong Liang,Yue Lin,Fan Xiao,Pengcheng Yi,Yanhong Xiong,Yao Dong,Hua Li,Lanran Fang,Hailin Liu,Jun Ying,Xifeng Wang

Cancer research 83:1280-1298 PubMed36799863

2023

Dysregulation of Mitochondrial Translation Caused by CBFB Deficiency Cooperates with Mutant PIK3CA and Is a Vulnerability in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Navdeep Malik,Young-Im Kim,Hualong Yan,Yu-Chou Tseng,Wendy du Bois,Gamze Ayaz,Andy D Tran,Laura Vera-Ramirez,Howard Yang,Aleksandra M Michalowski,Michael Kruhlak,Maxwell Lee,Kent W Hunter,Jing Huang

Cell death & disease 13:988 PubMed36418320

2022

COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yetong Feng,Jiayi Xu,Mengjiao Shi,Rongrong Liu,Lei Zhao,Xin Chen,Miaomiao Li,Yaping Zhao,Jiahui Chen,Wenjing Du,Pengfei Liu

Oxidative medicine and cellular longevity 2022:3187463 PubMed36425056

2022

Vaspin Attenuates Atrial Abnormalities by Promoting ULK1/FUNDC1-Mediated Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmin Zhu,Zhoushan Gu,Jiayu Shi,Chu Chen,Haixia Xu,Qi Lu

American journal of respiratory cell and molecular biology 68:103-115 PubMed36264759

2022

Intracellular Chloride Channels Regulate Endothelial Metabolic Reprogramming in Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mai M Alzaydi,Vahitha B Abdul-Salam,Harry J Whitwell,Giusy Russomanno,Angelos Glynos,Daria Capece,Gyorgy Szabadkai,Martin R Wilkins,Beata Wojciak-Stothard

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mitochondria Isolation Kit for Cultured Cells