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AB322033

Human CD4+ T Cell Isolation Kit

Human CD4+ T Cell Isolation Kit

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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
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Flow Cytometry - Human CD4+ T Cell Isolation Kit (AB322033)
  • Flow Cyt

Supplier Data

Flow Cytometry - Human CD4+ T Cell Isolation Kit (AB322033)

Untouched human CD4+ T cells were isolated from MNCs using the Human CD4+ T Cell Isolation Kit. Isolated cells were labeled with CD3-FITC and CD4-APC. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.

Key facts

アッセイ時間

30m

アッセイタイプ

Cell-based

Quantitative
交差種

Human

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

製品の詳細

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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.

  • This kit is designed to process 10 samples, up to 100 million cells per sample
  • Fast & easy workflow enriches human peripheral blood mononuclear cells (PBMCs) for CD4+ T cells in 30 minutes
  • No magnet, no column, no harsh chemicals
  • Microbubble enrichment maintains cell health and physiology

Mechanism of Action: Human CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-CD4+ T) cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8, CD11b, CD14, CD16, CD19, CD36, CD56, CD123, CD235a, and TCR-γδ.

製品内容

{ "values": { "1000000000Cellsvial": { "sellingSize": "1000000000 Cells/vial", "publicAssetCode":"ab322033-1000000000Cells", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"Separation Buffer", "number":"AB322033-CMP03", "productcode":"" }, { "size":"1 x 15.5 mL", "name":"BACS Streptavidin Microbubbles", "number":"AB322033-CMP01", "productcode":"" }, { "size":"1 x 1050 µL", "name":"Human CD4+ T Cell Biotin Antibody Cocktail", "number":"AB322033-CMP02", "productcode":"" }, { "size":"20 x 1 Vial", "name":"5 mL Tubes", "number":"AB322033-CMP04", "productcode":"" } ] } } }

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C

製品プロトコール

ターゲットの情報

See full target information CD4+ T Cell
websiteProtocolBooklet
en

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