AB322031

Human B Cell Isolation Kit

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The Human B Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched human B cells. Unwanted cells are depleted through negative selection.

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Flow Cytometry - Human B Cell Isolation Kit (AB322031)

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Supplier Data

Flow Cytometry - Human B Cell Isolation Kit (AB322031)

Untouched human B cells were isolated from PBMCs using the Human B Cell Isolation Kit. Isolated cells were labeled with CD3-FITC and CD19-APC/Fire750. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.

Key facts

アッセイ時間

30m

アッセイタイプ

Cell-based (quantitative)

交差種

Human

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

The Human B Cell Isolation Kit was developed with BACS™ Microbubbles for the enrichment of untouched human B cells. Unwanted cells are depleted through negative selection.

This kit can process up to 1 billion MNCs.
Fast & easy workflow enriches human mononuclear cells (MNCs) for B cells in 30 minutes
No magnet, no column, no harsh chemicals
Microbubble enrichment maintains cell health and physiology

Mechanism of Action: The Human B Cell Isolation Kit uses a negative selection method to remove unwanted cell populations and effectively enrich B cells. Non-B cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD2, CD3, CD14, CD15, CD16, CD36, CD43, CD56, CD57, CD123, CD161, CD235ab, and CD314.

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製品内容

{ "values": { "1000000000Cellsvial": { "sellingSize": "1000000000 Cells/vial", "publicAssetCode":"ab322031-1000000000Cells", "assetComponentDetails": [ { "size":"1 x 1050 µL", "name":"Human B Cell Biotin Antibody Cocktail", "number":"AB322031-CMP02", "productcode":"" }, { "size":"20 x 1 Vial", "name":"5 mL Tubes", "number":"AB322031-CMP04", "productcode":"" }, { "size":"1 x 10.5 mL", "name":"BACS Streptavidin Microbubbles", "number":"AB322031-CMP01", "productcode":"" }, { "size":"1 x 200 mL", "name":"Separation Buffer", "number":"AB322031-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

See full target information B Cell

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websiteProtocolBooklet

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Abcam product promise

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