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AB170197

HeLa Whole Cell RIPA Extract

HeLa Whole Cell RIPA Extract

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(0 Publication)

HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma.
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ELISA - HeLa Whole Cell RIPA Extract (AB170197)
  • ELISA

Supplier Data

ELISA - HeLa Whole Cell RIPA Extract (AB170197)

Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) using HeLa Whole Cell RIPA Extract.

Glucose 6 Phosphate Isomerase (G6PI) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574). A standard curve generated using the G6PI standard protein is shown (left image). G6PI signal using 2-fold serial dilutions of ab170197 starting at 50 μg/mL (right image). The G6PI concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 24.9 μg/mL.

ELISA - HeLa Whole Cell RIPA Extract (AB170197)
  • ELISA

Supplier Data

ELISA - HeLa Whole Cell RIPA Extract (AB170197)

mtTFA (TFAM) Human ELISA Kit (ab123455) using HeLa Whole Cell RIPA Extract.

Mitochondrial Transcription Factor A (mtTFA) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using mtTFA (TFAM) Human ELISA Kit (ab123455). A standard curve generated using the mtTFA standard protein is shown (left panel). mtTFA signal using 2-fold serial dilutions of ab170197 starting at 400 μg/mL is shown (right panel). The mtTFA concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 841 ng/mL.

Key facts

サンプルタイプ

Cell Lysate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected. Protein concentration of the extract was determined by BCA assay. This products is suitable for sandwich ELISAs, Immunoprecipitation, SDS-PAGE and Western Blotting.

Avoid freeze-thaw cycles with this product.

Concentration: Each unit contains a minimum of 300 μg at a lot-specific concentration. See label for concentration.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "2Unit": { "sellingSize": "2 Unit", "publicAssetCode":"ab170197-2Unit", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"HeLa Whole Cell RIPA Extract", "number":"AB170197-CMP01", "productcode":"" } ] } } }
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
-80°C
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製品プロトコール

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ターゲットの情報

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組合せ製品

Cell Lines & Lysates

AB150035

HeLa whole cell lysate

Western blot - HeLa whole cell lysate (AB150035)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Reagents, Controls & Accessories

AB64211

Antibody Diluent

  • 5
  • 1
Primary Antibodies

AB9482

HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)

  • 5
  • 20

Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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