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AB327040

Lyophilized Exosome Standard (A549 cell line)

Lyophilized Exosome Standard (A549 cell line)

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Lyophilized Exosome Standard (A549 cell line)(ab327040) contains purified exosomes (small-EVs) of lung carcinoma cell line (A549) origin. Available in 2x 100µg format. Suitable to use in the following applications:
- Control (spike-in) for EV quantification, protein marker analysis (ELISA, Western blot, Flow cytometry).
- Extraction and analysis of EV associated nucleic acids (qPCR, ddPCR, RNA seq) and NTA, fluorescent NTA (F-NTA).
- Single EV analysis by electron microscopy (TEM), Fluorescent microscopy.
- Number of particles in 100µg: > 1x1010
2 Images
Sandwich ELISA - Lyophilized Exosome Standard (A549 cell line) (AB327040)
  • sELISA

Supplier Data

Sandwich ELISA - Lyophilized Exosome Standard (A549 cell line) (AB327040)

Marker expression analysis performed by double sandwich ELISA

Size Exclusion Chromatography - Lyophilized Exosome Standard (A549 cell line) (AB327040)
  • Size Exclusion Chromatography

Supplier Data

Size Exclusion Chromatography - Lyophilized Exosome Standard (A549 cell line) (AB327040)

Size distribution profile of EVs measured by NTA

Key facts

アプリケーション

Size Exclusion Chromatography, sELISA

applications

製品の状態

Lyophilized

form

バッファー組成

Constituents: PBS, 6% Sucrose

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Size Exclusion Chromatography": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

製品の詳細

Purified exosomes (small EVs) and microvesicles (large EVs) are produced from cell-conditioned media prepared from cells sourced from a certified cell bank. Exosomes/small EVs (40–120 nm) are purified using a tangential flow filtration process, followed by size exclusion chromatography (SEC). Purified EVs are validated and characterized for overall protein content, marker expression (CD9, CD63, CD81), particle concentration and size distribution, and morphology (TEM).

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C

製品プロトコール

ターゲットの情報

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