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VEGFA peptide (ab46160)

Submit a review Q&A (1)References (3)
Western blot - VEGFA peptide (ab46160)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking

    製品の詳細

    関連製品

    特性

    Our Abpromise guarantee covers the use of ab46160 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Blocking - Blocking peptide for Anti-VEGFA antibody (ab46154)

    • 製品の状態

      Liquid

    • 備考

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    関連情報

    • 別名

      • Folliculostellate cell-derived growth factor
      • Glioma-derived endothelial cell mitogen
      • MGC70609
      • MVCD1
      • vascular endothelial growth factor
      • Vascular endothelial growth factor A
      • vascular endothelial growth factor A121
      • vascular endothelial growth factor A165
      • Vascular permeability factor
      • Vegf
      • VEGF A
      • VEGF-A
      • VEGF120
      • Vegfa
      • VEGFA_HUMAN
      • VPF
      see all

    • 機能

      Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

    • 組織特異性

      Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

    • 関連疾患

      Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

    • 配列類似性

      Belongs to the PDGF/VEGF growth factor family.

    • 細胞内局在

      Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    • Information by UniProt

    画像

    • Western blot - VEGFA peptide (ab46160)
      Western blot - VEGFA peptide (ab46160)
      All lanes : Anti-VEGFA antibody (ab46154) at 0.5 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A-431 whole cell lysate (ab7909)
      Lane 4 : HEK-293 whole cell lysate (ab7902)
      Lane 5 : Hep G2 whole cell lysate (ab7900)
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 9 : Jurkat whole cell lysate (ab7899) with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 10 : A-431 whole cell lysate (ab7909) with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 11 : HEK-293 whole cell lysate (ab7902) with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 12 : Hep G2 whole cell lysate (ab7900) with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 13 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with VEGFA peptide (ab46160) at 1 µg/ml
      Lane 14 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with VEGFA peptide (ab46160) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab46160 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab46160 は 3 報の論文で使用されています。

    • Dong D  et al. microRNA-646 inhibits angiogenesis of endothelial progenitor cells in pre-eclamptic pregnancy by targeting the VEGF-A/HIF-1a axis. Exp Ther Med 20:1879-1888 (2020). PubMed: 32782496
    • Hu XM  et al. Vascular Endothelial Growth Factor A Signaling Promotes Spinal Central Sensitization and Pain-related Behaviors in Female Rats with Bone Cancer. Anesthesiology 131:1125-1147 (2019). PubMed: 31567367
    • Han B  et al. Effects of tripterygium glycosides on restenosis following endovascular treatment. Mol Med Rep 13:4959-68 (2016). PubMed: 27108914

    レビューと Q&A

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    Question

    Dear technical team,

    Our customer purchased ab46160 and is going to dilute at 1μg/ml concentration for aliquot.
    But there is no information about storage buffer so, please let me know which buffer the customer can use for dilution.

    Best regards

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab46160 VEGF peptideis supplied in the followingbuffer:

    TK buffer with the following components:
    30 mM HEPES
    150 mM NaCl
    2 mM EDTA
    0.001% Tween 20
    At pH 6.75

    This is the buffer for the batch you have received, which according to our records is lot number 851217. The buffercan change betweenbatches, so I can recommend tocontact us again if you require this information for any future vials.

    Please note that we do not recommend to dilute the proteins, peptides or antibodies any further before storing them. The protein or peptide molecules can be less stable when storedat lower concentrations. Therefore, I can suggest to store the peptideas recommended on the datasheet and not to dilute itfirst so that the peptide remains stable and we can continue to provide our Abpromise guarantee.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

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