AB7403

Streptavidin (HRP)

Name: Streptavidin (HRP) (ab7403) | Abcam
Brand: Abcam Limited
SKU: AB7403
Rating: 5 (7 reviews)

(7 Reviews)

(214 Publications)

Streptavidin (HRP) is a Full Length native Streptavidin protein expressed in Streptomyces avidinii and conjuated with Horseradish peroxidase (HRP). ab7403 has >95% purity and suitable for western blot, ELISA, IHC, ICC, Dot blot and more.

- Used to detect biotin-conjugated primary antibodies
- Has less non-specific binding and less background signal compared to avidin
- Cited in over 170 publications

別名を表示する

Streptavidin

2 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

ELISA

Lab

ELISA - Streptavidin (HRP) (AB7403)

The wells were coated with Anti-Human IgG antibody [IG266] (ab200699) at 1μg/ml at 50μl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG3 (ab138703) was then added starting at 20 μg/ml and plasma/serum at 1 : 500 and gradually diluted 1 : 4, 50μl/well for 2h. ab201248 was then added at 1 : 10,000 dilution, 50μl/well for 2h. A HRP-streptavidin (ab7403) was used at 1 : 10,000 dilution for 1h.

Key facts

精製度

>95% SDS-PAGE

発現系

Native

アプリケーション

ELISA, IHC-P, IHC-Fr, IM, WB, ICC, Dot

applications

生物活性

Yes

生物学的活性

Binds to Biotin. Dissociation constant has not been measured.

標識

HRP

励起波長/蛍光波長

アクセッション番号

P22629

アニマルフリー

キャリアフリー

種

Streptomyces avidinii

バッファー組成

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/200000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/40000", "notes":"" } } }

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製品の詳細

Ensure the validity of your result using our Streptavidin (HRP) ab7403 to detect biotinylated primary antibodies in western blot, ELISA, IHC and more.

Check out our ELISA protocol for more information here.

Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.

This product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (ab142041) as a substrate for 30 minutes at room temperature.

A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Optimal titers for other applications should be determined by the researcher.

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":"Native","accessionNumber":"P22629","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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製品の性状

製品の状態

Liquid

補足情報

Streptavidin-HRP was prepared from chromatographically purified streptavidin.

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一般的な情報

機能

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

配列の類似性

Belongs to the avidin/streptavidin family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

See full target information Streptavidin

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文献 (214)

Recent publications for all applications. Explore the full list and refine your search

Pharmacological reports : PR 77:1309-1322 PubMed40553248

2025

The impact of muscarinic and mGlu receptors modulators on MK-801-induced impairments in NO-dependent processes both in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Grzegorz Burnat,Michał Santocki,Leszek Kalinowski,Joanna M Wierońska

International journal of molecular sciences 26: PubMed40362222

2025

Highly Potent Neutralizing Nanobodies Acting Against Chikungunya Virus Infection via Inhibiting Multiple Stages of the Viral Life Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Song,Guangcheng Fu,Jie Li,Zhengshan Chen,Ling Fu,Changming Yu,Li Qiang,Jiangfan Li,Ting Fang,Hongyu Yuan,Jianmin Li

Signal transduction and targeted therapy 10:139 PubMed40289109

2025

N-deglycosylation targeting chimera (DGlyTAC): a strategy for immune checkpoint proteins inactivation by specifically removing N-glycan.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Jiajia Wu,Weiqian Cao,Wei Zhang,Qi Wu,Yaxu Li,Yanrong Yang,Zezhi Shan,Zening Zheng,Xin Ge,Liang Lin,Ping Wang

EBioMedicine 113:105599 PubMed39955975

2025

An LNP-mRNA vaccine modulates innate cell trafficking and promotes polyfunctional Th1 CD4 T cell responses to enhance BCG-induced protective immunity against Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Lukeman,Hareth Al-Wassiti,Stewart A Fabb,Leonard Lim,Trixie Wang,Warwick J Britton,Megan Steain,Colin W Pouton,James A Triccas,Claudio Counoupas

eLife 13: PubMed39932490

2025

Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mie Suzuki Okutani,Shinya Okamura,Tang Gis,Hitomi Sasaki,Suni Lee,Akiho Kashiwabara,Simon Goto,Mai Matsumoto,Mayuko Yamawaki,Toshiaki Miyazaki,Tatsuya Nakagawa,Masahito Ikawa,Wataru Kamitani,Shiro Takekawa,Koichi Yamanishi,Hirotaka Ebina

Nature communications 16:1388 PubMed39910101

2025

A covalent peptide-based lysosome-targeting protein degradation platform for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Youmei Xiao,Zhuoying He,Wanqiong Li,Danhong Chen,Xiaoshuang Niu,Xin Yang,Wenxuan Zeng,Mengfan Wang,Yuzhen Qian,Ye Su,Feiyu Luo,Guanyu Chen,Juan Liu,Xinghua Sui,Xiuman Zhou,Yanfeng Gao

Nature communications 15:9603 PubMed39505901

2024

Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Negin Khosraviani,V Talya Yerlici,Jonathan St-Germain,Yi Yang Hou,Shi Bo Cao,Carla Ghali,Michael Bokros,Rehna Krishnan,Razqallah Hakem,Stephen Lee,Brian Raught,Karim Mekhail

mAbs 16:2416453 PubMed39400041

2024

GB18-06, a nanobody targeting GDF15, effectively alleviates weight loss and restores physical function in cachexia models.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Huang,Jinyong Wang,Xiling Wei,Hui Zhang,Wei Shang,Xiangling Zhang,Lanjiao Zhai,Xi Chen,Huiming Li,Suofu Qin

Brain and behavior 14:e70009 PubMed39236116

2024

Zuranolone therapy protects frontal cortex neurodevelopment and improves behavioral outcomes after preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Roisin A Moloney,Hannah K Palliser,Carlton L Pavy,Julia C Shaw,Jonathan J Hirst

eLife 13: PubMed39230574

2024

Quantitative mapping of proteasome interactomes and substrates using ProteasomeID.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandar Bartolome,Julia C Heiby,Domenico Di Fraia,Ivonne Heinze,Hannah Knaudt,Ellen Spaeth,Omid Omrani,Alberto Minetti,Maleen Hofmann,Joanna M Kirkpatrick,Therese Dau,Alessandro Ori

View all publications

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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Streptavidin (HRP)