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Recombinant Staphylococcus aureus Protein A (ab84187)

Submit a review Q&A (2)
SDS-PAGE - Recombinant Protein A (ab84187)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Staphylococcus aureus Protein A
      Protein A タンパク質・ペプチド 製品一覧

    • 精製度

      > 90 % SDS-PAGE.
      ab84187 is purified using conventional chromatography techniques.

    • 発現系

      Escherichia coli

    • アクセッション番号

      B3GGB2

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Staphylococcus aureus

      • 配列

        MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET

      • 領域

        37 to 469

    特性

    Our Abpromise guarantee covers the use of ab84187 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid

    • 備考

      Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.242% Tris, 10% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • gG-binding protein A
      • Immunoglobulin G binding protein A
      • SPA
      • Staphylococcal protein A

    • 関連性

      Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

    • 細胞内局在

      Secreted; cell wall.

    画像

    • SDS-PAGE - Recombinant Protein A (ab84187)
      SDS-PAGE - Recombinant Protein A (ab84187)
      15% SDS-PAGE showing ab84187 at approximately 48.1kDa (3µg).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab84187 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab84187 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Dear technical support team: This customer raises an enquiry about ab7399 (Protein A protein) and ab84187 (Protein A protein), she wants to know which one is more suitable to bind with ab9582 (Anti-hCG beta antibody [5H4-E2]), could you please help this customer to solve the problem? Thanks for your kindly help Best regards

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    Answer

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab9582 (Anti-hCG beta antibody [5H4-E2]), has not been tested for IP yet, though the isotype has been determined (IgG1).

    Normally, we would suggest using Protein G for mouse IgG1 antibodies; whilst for moue IgG2a and mouse IgG2b can be used with both Protein A and G. Please could you advise your customer to take a look at this site for further information:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11385

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Question

    - protein G (full length)
    - protein G (full length) + His tag ....... ab7458.

    - protein A (full length) ....... ab117657.
    - protein A (full length) + His tag

    Read More
    Answer

    J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
    - une protéine G (full length sans tag) en complément de la référence ab7458 (protéine G, full length, His tag),
    - une protéine A (full length avec His-tag) en complément de la référence ab117657 (protéine A, full length, sans tag)

    Je vous contacterai de nouveau avec les informations que mes collègues m'auront fournies.

    Merci pour votre patience et bon week-end.

    Read More

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