Recombinant S. japonicum GST protein (ab70456)

Thank you for contacting Abcam.

The GST protein, ab70456 should work fine in a western blot, although since we have not technically tested it in this application, then we cannot guarantee that it will work. Therefore you could take advantage of our 100% Abreview testing discount program:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



I would recommend the following primary antibody for use in western blot:

https://www.abcam.com/GST-antibody-ab9085.html



If there is anything else I can help you with, or if you would like to take advantage of out testing discount offer, then please just let me know.

I am very glad to hear that our anti-Beta Actin loading control has worked so well for you.

In regards to a loading control for your current needs, Beta Actin would only be present in serum if shearing of cells had occurred during collection so this would not make a viable control. I have come across data stating that tranferrin and alpha anti-Trypsin are usually expressed at levels ˜2.5mg/ml and ˜1.3mg/ml respectively. However, as these may vary in samples, it may be best to add a purified peptide such a GST to your sample and stain against that.

We do offer antibodies against Transferrin, alpha anti-Trypsin and GST as well as purified GST peptides.

GST (ab70456):https://www.abcam.com/GST-protein-ab70456.html

anti-GST antibody (ab92):https://www.abcam.com/GST-antibody-3G10-1B3-ab92.html

anti-alpha-anti-Trypsin antibody (ab7633):https://www.abcam.com/alpha-1-Antitrypsin-antibody-ab7633.html

anti-Tranferrin antibody (ab1223):https://www.abcam.com/Transferrin-antibody-ab1223.html



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.