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Recombinant S. cerevisiae GAL4 protein (ab81879)

Reviews (1)Q&A (3)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE, Functional Studies

製品の詳細

  • 製品名

    Recombinant S. cerevisiae GAL4 protein
    GAL4 タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % SDS-PAGE.
    ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.

  • 発現系

    Escherichia coli

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Saccharomyces cerevisiae

    • 領域

      1 to 147

  • 製品の詳細

    Recombinant S. cerevisiae GAL4 protein

特性

Our Abpromise guarantee covers the use of ab81879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態

    Liquid

  • 備考


    Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • Antigen NY CO 27
    • Antigen NY-CO-27
    • Antigen NYCO27
    • GAL 4
    • Gal-4
    • GAL4
    • Galectin 4
    • Galectin-4
    • Galectin4
    • Homo sapiens galectin4 mRNA complete cds
    • L 36 lactose binding protein
    • L-36 lactose-binding protein
    • L36 lactose binding protein
    • L36LBP
    • Lactose binding lectin 4
    • Lactose-binding lectin 4
    • Lectin galactoside binding soluble 4
    • LEG4_HUMAN
    • LGALS4
    see all

  • 機能

    Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

  • 配列類似性

    Contains 2 galectin domains.

  • ドメイン

    Contains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab81879 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab81879 は論文での使用が確認できていません。

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

An abreview for ab81879 - Recombinant S. cerevisiae GAL4 protein

 Excellent
Abreviews
abreview image
Application
Western blot
Application: Western blot
Gel Running Conditions: Reduced Denaturing (12% gel)
Blocking step: 5% skim milk as blocking agent for 1 hour at RT
Primary antibody:
Abcam antibody ab135397 Anti-GAL4 antibody [15-6E10A7]
Dilution: 1/1000 Diluent: TBS + 0.05% sodium azide Incubation: 1 hour at RT
Secondary antibody:
Alkaline Phosphatase AffiniPure Donkey Anti-Mouse IgG (H+L) Dilution: 1/4000 Incubation: 1 hour at RT
Detection
Detection method: BCIP/NBT Exposure: 2 minutes
Bands
Specific: 18.6 kDa
Additional notes: there is an additional faint band at about 37 kDa, perhaps due to dimerization
Image: MW Ladder: SuperMarker2700 (5ul)
lane #1: 100 ng of ab81879 lane #2: 50 ng of ab81879 lane #3: 25 ng of ab81879
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 24 2021

Question

BTW, just to be sure - in the figure that they sent us, the protein that we are talking about is gal4-147? What is the gal4-AH protein?

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Answer

The GAL4 –AH (P1018) has a fragment 1-147 aa and an additiona alpha helix sequence (denoted AH).

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Question

Here are a few questions regarding ab81879:

We have few questions regarding this product that will enable us to decide if we order the product :

1. Does the gal4 protein contain any tag? If so, where is it fused (N or C terminal parts)?
2. What is the helix that is attached to the protein? Where is it fused (N or C terminal parts)?
3. Can we use this protein in in vitro binding assay (binding of the gal4 protein to a DNA molecule)? Did abcam tried it in in vitro binding assays? Do they have references for papers that used the protein that they sell?

Please send us this information ASAP so we can take out an order this week.

Read More
Answer

Please find the answer from the lab and the attached data:

"The protein contains no tag but an alpha-helix peptide on its C-terminus. Yes, the protein is active for gel shift experiment. Attached please find the gel shift experimental data and image as well as the product sheet for GAL4 fused proteins."

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Question

What is the purpose of the alpha helix on thisGAL4 protein?

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Answer

Thank you for contacting us.

In the absence of the alpha helix, the GAL4 protein willbind to DNA but will not activate transcription. The alpha helix allows the protein complex to activate genes involved in galactose metabolism.


I hope this helps, please let me know if you need any additional information or assistance.

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