Recombinant Rat SCF Protein (Active) (ab290071)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
製品の詳細
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製品名
Recombinant Rat SCF Protein (Active)
SCF タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of TF-1 cells.
ED50 is ≤ 15.72 ng/ml, corresponding to a specific activity of 6.4 x 104units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Rat -
領域
26 to 189 -
配列の追加情報
N-terminal glycine.
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製品の詳細
Recombinant Rat SCF Protein
特性
Our Abpromise guarantee covers the use of ab290071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product
関連情報
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別名
- C kit ligand
- C-kit ligand
- Ckit ligand
see all -
機能
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. -
関連疾患
Hyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
配列類似性
Belongs to the SCF family. -
発生段階
Acts in the early stages of hematopoiesis. -
翻訳後修飾
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
細胞内局在
Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. - Information by UniProt
画像
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Functional Studies - Recombinant Rat SCF Protein (ab290071)
Fully biologically active determined by the dose dependent proliferation of TF-1 cells.
ED50 is ≤ 15.72 ng/ml, corresponding to a specific activity of 6.4 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3447558-1.
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SDS-PAGE - SCF protein (ab290071)SDS-PAGE analysis of ab290071 -
Mass Spectrometry - SCF protein (ab290071)Mass determination by ESI-TOF.
Predicted MW is 18396.10Da. (+/- 10 Da by ESI-TOF). Observed MW is 18397.53 Da. Additional masses are due to residual O-glycans.
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HPLC - SCF protein (ab290071)HPLC analysis of ab290071
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab290071 は論文での使用が確認できていません。
