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AB307410

Recombinant Rat M-CSF Protein (Active)

Recombinant Rat M-CSF Protein (Active)

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Recombinant Rat M-CSF Protein (Active) is a Rat Fragment protein, in the 33 to 190 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS.

別名を表示する

Csfm, Csf1, Macrophage colony-stimulating factor 1, CSF-1, MCSF, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF

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Biological Activity - Recombinant Rat M-CSF Protein (Active) (AB307410)
  • Biological Activity

Lab

Biological Activity - Recombinant Rat M-CSF Protein (Active) (AB307410)

Fully biologically active determined by the dose dependent proliferation of M-NFS-60 cells. ED50 is ≤ 1.8 ng/ml, corresponding to a specific activity of 5.5 x 10^5 units/mg.

Mass Spectrometry - Recombinant Rat M-CSF Protein (Active) (AB307410)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Rat M-CSF Protein (Active) (AB307410)

Mass determination by ESI-TOF. Predicted MW is 18505.07 Da (+/-10 Da by ESI-TOF). Observed MW is 18506.60 Da.

SDS-PAGE - Recombinant Rat M-CSF Protein (Active) (AB307410)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Rat M-CSF Protein (Active) (AB307410)

SDS-PAGE analysis of ab307410

HPLC - Recombinant Rat M-CSF Protein (Active) (AB307410)
  • HPLC

Supplier Data

HPLC - Recombinant Rat M-CSF Protein (Active) (AB307410)

HPLC analysis of ab307410

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

Mass Spec, SDS-PAGE, HPLC, FuncS

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by the dose dependent proliferation of M-NFS-60 cells. ED50 is ≤ 1.8 ng/ml, corresponding to a specific activity of 5.5 x 105 units/mg.

アクセッション番号

Q8JZQ0

アニマルフリー

Yes

キャリアフリー

No

種

Rat

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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配列情報

[{"sequence":"EVSEHCSHMIGNGHLQILQQLIDSQMETACLIEYKFVDQEQLDDPVCYLKKAFVLVQVIIEETMRFKDNTPNANATERLQELSMKLNSCFIKDYKEQNEACVQTYKESPLRLLEKIKNFFNETKNFLEKDWNIFSKNCNDSFAKCSSRDVVTKPDCNC","proteinLength":"Fragment","predictedMolecularWeight":"18.5 kDa","actualMolecularWeight":"18.5 kDa","aminoAcidEnd":190,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8JZQ0","tags":[]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF's role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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製品の性状

製品の状態

Lyophilized

補足情報

SDS-PAGE >= 95%

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一般的な情報

機能

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).

翻訳後修飾

N-glycosylated.. O-glycosylated; contains chondroitin sulfate.

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製品プロトコール

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ターゲットの情報

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
See full target information Csf1
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