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AB282395

Recombinant rat IL-4 protein (Active)

Recombinant rat IL-4 protein (Active)

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Recombinant rat IL-4 protein (Active) is a Rat Full Length protein, in the 25 to 147 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

別名を表示する

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

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Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)

Functional analysis of ab282395.

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3393276-4

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)

Mass determination by ESI-TOF.

Predicted MW is 13820.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 13821.71

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)

SDS-page analysis of ab282395.

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)
  • HPLC

Supplier Data

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)

HPLC analysis of ab282395.

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

SDS-PAGE, HPLC, Mass Spec, FuncS

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

アクセッション番号

P20096

アニマルフリー

Yes

キャリアフリー

No

種

Rat

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS","proteinLength":"Full Length","predictedMolecularWeight":"13.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":147,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20096","tags":[]}]
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出荷温度及び保存条件

出荷温度
Ambient - Can Ship with Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

配列の類似性

Belongs to the IL-4/IL-13 family.

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製品プロトコール

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ターゲットの情報

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Abcam product promise

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