AB233668

Recombinant rat IL-3 protein (Active)

Bioactive
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Recombinant rat IL-3 protein (Active) is a Rat Full Length protein, in the 27 to 166 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

別名を表示する

Il-3, Il3, Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF

2 Images

Functional Studies - Recombinant rat IL-3 protein (Active) (AB233668)

FuncS

Supplier Data

Functional Studies - Recombinant rat IL-3 protein (Active) (AB233668)

ab233668 induced proliferation of NFS-60 cells.

SDS-PAGE - Recombinant rat IL-3 protein (Active) (AB233668)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-3 protein (Active) (AB233668)

1 μg ab233668 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.

Lane 1 : Non-reducing conditions.

Lane 2 : Reducing conditions.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<1 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

NFS-60 cell proliferation: ED₅₀ ≤ 10 ng/ml (≥ 1.0 x 10⁴ units/mg).

アクセッション番号

P04823

アニマルフリー

キャリアフリー

種

Rat

再構成

Reconstitute at 0.1 mg/mL in water

バッファー組成

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MISDRGSDAHHLLRTLDCRTIALEILVKLPYPQVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTTDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC","proteinLength":"Full Length","predictedMolecularWeight":"16.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":166,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

True

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 34183475). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (By similarity). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (By similarity).

配列の類似性

Belongs to the IL-3 family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Il3

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Recombinant rat IL-3 protein (Active)