Recombinant Rat EGF Protein (Active) (ab290070)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, MS, HPLC
製品の詳細
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製品名
Recombinant Rat EGF Protein (Active)
EGF タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of Balb/c-3T3 cells.
ED50 is ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 107units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Rat -
配列
NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWW KLR -
領域
974 to 1026 -
配列の追加情報
N-terminal glycine.
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製品の詳細
Recombinant Rat EGF Protein
特性
Our Abpromise guarantee covers the use of ab290070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Mass Spectrometry
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product
関連情報
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別名
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
機能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
組織特異性
Expressed in kidney, salivary gland, cerebrum and prostate. -
関連疾患
Hypomagnesemia 4 -
配列類似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻訳後修飾
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - Recombinant Rat EGF Protein (ab290070)
Fully biologically active determined by the dose dependent proliferation of Balb/c-3T3 cells.
ED50 is ≤ 0.03 ng/ml, corresponding to a specific activity of 3.3 x 107 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3447557-2.
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SDS-PAGE - EGF protein (ab290070)SDS-PAGE analysis of ab290070 -
Mass Spectrometry - EGF protein (ab290070)Mass determination by ESI-TOF.
Predicted MW is 6208.04 Da. (+/- 10 Da by ESI-TOF). Observed MW is 6051.71 Da.
M -174 Da Due to Arginine truncation
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HPLC - EGF protein (ab290070)HPLC analysis of ab290070
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab290070 は論文での使用が確認できていません。
